Influence of Disk Preparation on Detection of Metallo-β-Lactamase-Producing Isolates by the Combined Disk Assay

ABSTRACT The combined disk assay has been used for detection of metallo-β-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many β-lactam/metallo-β-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 β-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.

[1]  A. Peleg,et al.  Phenotypic Detection of Carbapenem-Susceptible Metallo-β-Lactamase-Producing Gram-Negative Bacilli in the Clinical Laboratory , 2006, Journal of Clinical Microbiology.

[2]  G. Daikos,et al.  Evaluation of imipenem/imipenem+EDTA disk method for detection of metallo-beta-lactamase-producing Klebsiella pneumoniae isolated from blood cultures. , 2006, Microbial drug resistance.

[3]  Y. Ishii,et al.  Evaluation of dipicolinic acid for detection of IMP- or VIM- type metallo-beta-lactamase-producing Pseudomonas aeruginosa clinical isolates. , 2005, Diagnostic microbiology and infectious disease.

[4]  G. Rossolini,et al.  Novel Acquired Metallo-β-Lactamase Gene, blaSIM-1, in a Class 1 Integron from Acinetobacter baumannii Clinical Isolates from Korea , 2005, Antimicrobial Agents and Chemotherapy.

[5]  A. Viale,et al.  Sensitive EDTA-Based Microbiological Assays for Detection of Metallo-β-Lactamases in Nonfermentative Gram-Negative Bacteria , 2005, Journal of Clinical Microbiology.

[6]  D. Church,et al.  Detection of Pseudomonas aeruginosa Producing Metallo-β-Lactamases in a Large Centralized Laboratory , 2005, Journal of Clinical Microbiology.

[7]  Ronald N. Jones,et al.  Molecular Characterization of a β-Lactamase Gene, blaGIM-1, Encoding a New Subclass of Metallo-β-Lactamase , 2004, Antimicrobial Agents and Chemotherapy.

[8]  J. Wu,et al.  Comparison of the double-disk, combined disk, and Etest methods for detecting metallo-beta-lactamases in gram-negative bacilli. , 2004, Diagnostic microbiology and infectious disease.

[9]  Ronald N. Jones,et al.  Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme. , 2002, The Journal of antimicrobial chemotherapy.

[10]  G. Rossolini,et al.  Simple Microdilution Test for Detection of Metallo-β-Lactamase Production in Pseudomonas aeruginosa , 2002, Journal of Clinical Microbiology.

[11]  H. Shin,et al.  Imipenem-EDTA Disk Method for Differentiation of Metallo-β-Lactamase-Producing Clinical Isolates of Pseudomonas spp. and Acinetobacter spp , 2002, Journal of Clinical Microbiology.

[12]  A. Bolmström,et al.  Evaluation of a New Etest for Detecting Metallo-β-Lactamases in Routine Clinical Testing , 2002, Journal of Clinical Microbiology.

[13]  P. Nordmann,et al.  Emerging carbapenemases in Gram-negative aerobes. , 2002, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[14]  D. Yong,et al.  Modified Hodge and EDTA-disk synergy tests to screen metallo-beta-lactamase-producing strains of Pseudomonas and Acinetobacter species. , 2001, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[15]  Y. Arakawa,et al.  Convenient Test for Screening Metallo-β-Lactamase-Producing Gram-Negative Bacteria by Using Thiol Compounds , 2000, Journal of Clinical Microbiology.

[16]  F. Yoshimura,et al.  Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance , 1994, Antimicrobial Agents and Chemotherapy.