Encapsulation of Chondrocytes in High-Stiffness Agarose Microenvironments for In Vitro Modeling of Osteoarthritis Mechanotransduction

[1]  D. L. Zignego,et al.  The mechanical microenvironment of high concentration agarose for applying deformation to primary chondrocytes. , 2014, Journal of biomechanics.

[2]  J. Hilmer,et al.  Candidate mediators of chondrocyte mechanotransduction via targeted and untargeted metabolomic measurements. , 2014, Archives of biochemistry and biophysics.

[3]  Joshua T. Morgan,et al.  Substratum stiffness and latrunculin B modulate the gene expression of the mechanotransducers YAP and TAZ in human trabecular meshwork cells. , 2013, Experimental eye research.

[4]  D. Fyhrie,et al.  A comparison of cartilage stress-relaxation models in unconfined compression: QLV and stretched exponential in combination with fluid flow , 2013, Computer methods in biomechanics and biomedical engineering.

[5]  P. Kumta,et al.  Early differentiation patterning of mouse embryonic stem cells in response to variations in alginate substrate stiffness , 2013, Journal of biological engineering.

[6]  Gary Siuzdak,et al.  Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database , 2013, Nature Protocols.

[7]  F. Guilak,et al.  Depth-dependent anisotropy of the micromechanical properties of the extracellular and pericellular matrices of articular cartilage evaluated via atomic force microscopy. , 2013, Journal of biomechanics.

[8]  S. Chien,et al.  Matrix Stiffness Regulates Endothelial Cell Proliferation through Septin 9 , 2012, PloS one.

[9]  M. Knight,et al.  Cell mechanics, structure, and function are regulated by the stiffness of the three-dimensional microenvironment. , 2012, Biophysical journal.

[10]  Benjamin J Ellis,et al.  Finite element prediction of cartilage contact stresses in normal human hips , 2012, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[11]  F. Guilak,et al.  A biomechanical role for perlecan in the pericellular matrix of articular cartilage. , 2012, Matrix biology : journal of the International Society for Matrix Biology.

[12]  F. Mallein-Gerin,et al.  Dynamic Compression of Chondrocyte-Agarose Constructs Reveals New Candidate Mechanosensitive Genes , 2012, PloS one.

[13]  Cristina Ruiz-Romero,et al.  Osteoarthritis: Metabolomic characterization of metabolic phenotypes in OA , 2012, Nature Reviews Rheumatology.

[14]  G. Ateshian,et al.  Transient Supplementation of Anabolic Growth Factors Rapidly Stimulates Matrix Synthesis in Engineered Cartilage , 2011, Annals of Biomedical Engineering.

[15]  C P Neu,et al.  Polymer Mechanics as a Model for Short-Term and Flow-Independent Cartilage Viscoelasticity. , 2011, Materials science & engineering. C, Materials for biological applications.

[16]  Horacio D Espinosa,et al.  Substrate stiffness regulates extracellular matrix deposition by alveolar epithelial cells. , 2011, Research and reports in biology.

[17]  G. Ateshian,et al.  Response of engineered cartilage to mechanical insult depends on construct maturity. , 2010, Osteoarthritis and cartilage.

[18]  F. Guilak,et al.  Spatial mapping of the biomechanical properties of the pericellular matrix of articular cartilage measured in situ via atomic force microscopy. , 2010, Biophysical journal.

[19]  Liming Bian,et al.  Passaged adult chondrocytes can form engineered cartilage with functional mechanical properties: a canine model. , 2010, Tissue engineering. Part A.

[20]  D. Fyhrie,et al.  Enzymatic digestion of articular cartilage results in viscoelasticity changes that are consistent with polymer dynamics mechanisms , 2009, Biomedical engineering online.

[21]  F. Mallein-Gerin,et al.  Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures , 2009, Nature Protocols.

[22]  D P Fyhrie,et al.  Cartilage stress-relaxation is affected by both the charge concentration and valence of solution cations. , 2009, Osteoarthritis and cartilage.

[23]  Jason A. Burdick,et al.  Differential maturation and structure-function relationships in mesenchymal stem cell- and chondrocyte-seeded hydrogels. , 2009, Tissue engineering. Part A.

[24]  David P Fyhrie,et al.  Cartilage stress-relaxation proceeds slower at higher compressive strains. , 2009, Archives of biochemistry and biophysics.

[25]  C. Poole,et al.  Articular chondrocytes express connexin 43 hemichannels and P2 receptors – a putative mechanoreceptor complex involving the primary cilium? , 2009, Journal of anatomy.

[26]  L. Bian,et al.  Physiologic deformational loading does not counteract the catabolic effects of interleukin-1 in long-term culture of chondrocyte-seeded agarose constructs. , 2008, Journal of biomechanics.

[27]  D. D’Lima,et al.  Rho kinase-dependent CCL20 induced by dynamic compression of human chondrocytes. , 2008, Arthritis and rheumatism.

[28]  A. Grodzinsky,et al.  Shear- and Compression-induced Chondrocyte Transcription Requires MAPK Activation in Cartilage Explants* , 2008, Journal of Biological Chemistry.

[29]  Afshin Khalafi,et al.  Mechanotransduction of bovine articular cartilage superficial zone protein by transforming growth factor beta signaling. , 2007, Arthritis and rheumatism.

[30]  Philip E. Martin,et al.  Mechanical power and efficiency of level walking with different stride rates , 2007, Journal of Experimental Biology.

[31]  Ying Zhang,et al.  HMDB: the Human Metabolome Database , 2007, Nucleic Acids Res..

[32]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.

[33]  Farshid Guilak,et al.  Osteoarthritic changes in the biphasic mechanical properties of the chondrocyte pericellular matrix in articular cartilage. , 2005, Journal of biomechanics.

[34]  Farshid Guilak,et al.  Alterations in the mechanical properties of the human chondrocyte pericellular matrix with osteoarthritis. , 2003, Journal of biomechanical engineering.

[35]  Gerard A Ateshian,et al.  Experimental verification of the roles of intrinsic matrix viscoelasticity and tension-compression nonlinearity in the biphasic response of cartilage. , 2003, Journal of biomechanical engineering.

[36]  P. Gennes.  Relaxation Anomalies in Linear Polymer Melts , 2002 .

[37]  Y. Benjamini,et al.  THE CONTROL OF THE FALSE DISCOVERY RATE IN MULTIPLE TESTING UNDER DEPENDENCY , 2001 .

[38]  G A Ateshian,et al.  Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. , 2000, Journal of biomechanical engineering.

[39]  G. Fredrickson.  The theory of polymer dynamics , 1996 .

[40]  H. Mankin,et al.  Chondrocyte survival in cryopreserved osteochondral articular cartilage , 1996, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[41]  Helen Muir,et al.  The chondrocyte, architect of cartilage. Biomechanics, structure, function and molecular biology of cartilage matrix macromolecules , 1995, BioEssays : news and reviews in molecular, cellular and developmental biology.

[42]  L. Bonassar,et al.  The role of cartilage streaming potential, fluid flow and pressure in the stimulation of chondrocyte biosynthesis during dynamic compression. , 1995, Journal of biomechanics.

[43]  S. Pérez,et al.  Modelling studies of solvent effects on the conformational stability of agarobiose and neoagarobiose and their relationship to agarose. , 1989, International journal of biological macromolecules.

[44]  Ronald N. Bracewell,et al.  The Fourier Transform and Its Applications , 1966 .

[45]  D. A. Lee,et al.  Quantification of mRNA using real-time PCR and Western blot analysis of MAPK events in chondrocyte/agarose constructs. , 2011, Methods in molecular biology.

[46]  John F. Bolton,et al.  Chondrocyte deformation within compressed agarose constructs at the cellular and sub-cellular levels. , 2000, Journal of biomechanics.