In vitro influence of 520 nm diode laser irradiation on red blood cell spontaneous aggregation studied by optical tweezers and light microscopy

The laser has biomodulation effects on blood properties thus could regulate the microcirculation. The laser-RBC interaction mechanism is unclear, whereas most studies provide statistical results and lack detailed observations of laser irradiation effects on blood rheology. This study is designated to probe the in vitro effects of 520 nm diode laser irradiation on red blood cells (RBCs) mutual interaction properties in spontaneous aggregation process in autologous plasma by optical tweezers (OTs), with an attempt to reveal the laser-RBC interaction outcomes at a single-cell level. The results preliminarily show that though the laser irradiation statistically inhibited the increase of the size of RBC aggregates compared with the non-irradiating group, the aggregation force between single RBCs increased slightly with the time of irradiation.

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