IMPEDANCE IMAGING FOR HYBRID BIOSENSOR APPLICATIONS

The impedance characteristics of individual cell / electrode systems are used to monitor cellular viability, position, adhesion, and response to external stimuli in hybrid biosensor applications. A planar microelectrode array consisting of 36 platinized iridium electrodes (10 μm diameter) is used as a substrate for the culture of mammalian cells. Electrode impedance is monitored across the array as different environmental factors are changed. Maps of electrode impedance have been shown to correlate directly to cell positioning over an electrode and general cellular viability. Exposure to a well known voltage-gated Na + channel blocker (tetrodotoxin) provided significant cellular response as compared to control electrodes without cells. The effective use of small electrodes (10 μm diameter) to study single cell / electrode interactions has been demonstrated.

[1]  Patricia Connolly,et al.  Microelectronic and Nanoelectronic Interfacing Techniques for Biological Systems , 1992 .

[2]  D. Lawrence,et al.  Measurement of macrophage adherence and spreading with weak electric fields. , 1990, Journal of immunological methods.

[3]  Thomas M. McKenna,et al.  Enabling Technologies for Cultured Neural Networks , 1994 .

[4]  Ivar Giaever,et al.  A Whole Cell Biosensor Based On Cell-substrate Interactions , 1990, [1990] Proceedings of the Twelfth Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[5]  G. Kovacs,et al.  A thermal signal generator probe for the study of neural thermal transduction , 1994, IEEE Transactions on Biomedical Engineering.

[6]  G. Gross.  Simultaneous Single Unit Recording in vitro with a Photoetched Laser Deinsulated Gold Multimicroelectrode Surface , 1979, IEEE Transactions on Biomedical Engineering.

[7]  J. Nicholls.  From neuron to brain , 1976 .

[8]  Ivar Giaever,et al.  Use of Electric Fields to Monitor the Dynamical Aspect of Cell Behavior in Tissue Culture , 1986, IEEE Transactions on Biomedical Engineering.

[9]  C.R. Keese,et al.  A biosensor that monitors cell morphology with electrical fields , 1994, IEEE Engineering in Medicine and Biology Magazine.

[10]  D. Stenger,et al.  Comparative electrophysiological properties of NG108-15 cells in serum-containing and serum-free media , 1993, Neuroscience Letters.

[11]  I. Giaever,et al.  Monitoring electropermeabilization in the plasma membrane of adherent mammalian cells. , 1993, Biophysical journal.

[12]  M. Nirenberg,et al.  Synapse formation between two clonal cell lines. , 1977, Science.