Inhibition of RNA synthesis by actinomycin D: Characteristic dose‐response of different RNA species

A quantitative dose‐response study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine. The 45S precursor of ribosomal RNA, 5S ribosomal RNA, transfer RNA and various sized fractions of heterogeneous nuclear RNA were extracted from the appropriate subcellular fractions and resolved by acrylamide gel electrophoresis. Polyribosomal messenger RNA was also studied. Over the range of actinomycin concentrations employed cellular uptake of the drug is proportional to external concentration, and maximal inhibition of RNA synthesis is generally achieved within one hour after exposure.

[1]  N. Craig.  On the regulation of the synthesis of ribosomal proteins in L-cells. , 1971, Journal of molecular biology.

[2]  R. Perry,et al.  Evolution of the transcription unit of ribosomal RNA. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[3]  B. Mccarthy,et al.  Characterization of RNA molecules restricted to the nucleus in mouse L‐cells , 1970, Journal of cellular physiology.

[4]  C. Woese,et al.  Transcriptional Mapping: Functional Organization of the Ribosomal and Transfer Ribonucleic Acid Cistrons in the Bacillus subtilis Genome , 1969, Journal of bacteriology.

[5]  E. Kano,et al.  CELL KILLING BY ACTINOMYCIN D IN RELATION TO THE GROWTH CYCLE OF CHINESE HAMSTER CELLS , 1969, The Journal of cell biology.

[6]  C. Woese,et al.  "Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Darnell,et al.  tRNA synthesis in HeLa cells: a precursor to tRNA and the effects of methionine starvation on tRNA synthesis. , 1969, Journal of molecular biology.

[8]  O. Miller,et al.  Visualization of Nucleolar Genes , 1969, Science.

[9]  A. Lima-de-faria.  Handbook of molecular cytology , 1969 .

[10]  H. Bremer,et al.  RNA chain growth-rate in Escherichia coli. , 1968, Journal of molecular biology.

[11]  R. Perry,et al.  Persistent synthesis of 5S RNA when production of 28S and 18S ribosomal RNA is inhibited by low doses of actinomycin D , 1968, Journal of cellular physiology.

[12]  R. Perry,et al.  Messenger RNA-protein complexes and newly synthesized ribosomal subunits: analysis of free particles and components of polyribosomes. , 1968, Journal of molecular biology.

[13]  K. Sakamoto,et al.  AGE‐DEPENDENT TOXIC PROPERTIES OF ACTINOMYCIN D AND X‐RAYS IN CULTURED CHINESE HAMSTER CELLS , 1968 .

[14]  S. Penman,et al.  Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells. , 1968, Journal of molecular biology.

[15]  D M Crothers,et al.  Studies of the binding of actinomycin and related compounds to DNA. , 1968, Journal of molecular biology.

[16]  J. Gurdon,et al.  Attachment of Rapidly Labelled RNA to Polysomes in the Absence of Ribosomal RNA Synthesis during Normal Cell Differentiation , 1967, Nature.

[17]  S. Spiegelman,et al.  Electrophoretic separation of viral nucleic acids on polyacrylamide gels. , 1967, Journal of molecular biology.

[18]  R. Perry.  The nucleolus and the synthesis of ribosomes. , 1965, Progress in nucleic acid research and molecular biology.

[19]  B. Mccarthy,et al.  Evidence for ribonucleic acid molecules restricted to the cell nucleus. , 1967, Biochemistry.

[20]  Mortimer M. Elkind,et al.  The radiobiology of cultured mammalian cells , 1967 .

[21]  S. Penman,et al.  Methylation and processing of ribosomal RNA in HeLa cells. , 1966, Journal of molecular biology.

[22]  J. Richardson.  The binding of RNA polymerase to DNA. , 1966, Journal of molecular biology.

[23]  M. Zalokar.  [STUDIES OF THE FORMATION OF RIBONUCLEIC ACID AND PROTEINS IN INSECTS]. , 1965, Revue suisse de zoologie; annales de la Societe zoologique suisse et du Museum d'histoire naturelle de Geneve.

[24]  R. Perry,et al.  Hybridization of Rapidly Labeled Nuclear Ribonucleic Acids , 1964, Science.

[25]  A. Ficq.  Effets de l'actinomycine D et de la puromycine sur le métabolisme de l'oocyte en croissance Étude autoradiographique , 1964 .

[26]  T. Staehelin,et al.  Ribosomal Aggregate Engaged in Protein Synthesis: Characterization of the Ergosome , 1963, Nature.

[27]  R. Perry,et al.  THE CELLULAR SITES OF SYNTHESIS OF RIBOSOMAL AND 4S RNA. , 1962, Proceedings of the National Academy of Sciences of the United States of America.