Issues in modern bone histomorphometry.

This review reports on proceedings of a bone histomorphometry session conducted at the Fortieth International IBMS Sun Valley Skeletal Tissue Biology Workshop held on August 1, 2010. The session was prompted by recent technical problems encountered in conducting histomorphometry on bone biopsies from humans and animals treated with anti-remodeling agents such as bisphosphonates and RANKL antibodies. These agents reduce remodeling substantially, and thus cause problems in calculating bone remodeling dynamics using in vivo fluorochrome labeling. The tissue specimens often contain few or no fluorochrome labels, and thus create statistical and other problems in analyzing variables such as mineral apposition rates, mineralizing surface and bone formation rates. The conference attendees discussed these problems and their resolutions, and the proceedings reported here summarize their discussions and recommendations.

[1]  F. Melsen,et al.  Missing observations in bone histomorphometry on osteoporosis: implications and suggestions for an approach. , 1999, Bone.

[2]  R. Recker,et al.  The label escape error: comparison of measured and theoretical fraction of total bone-trabecular surface covered by single label in normals and patients with osteoporosis. , 1986, Bone.

[3]  M P Akhter,et al.  Transmenopausal changes in the trabecular bone structure. , 2007, Bone.

[4]  D. Burr,et al.  Morphological Assessment of Basic Multicellular Unit Resorption Parameters in Dogs Shows Additional Mechanisms of Bisphosphonate Effects on Bone , 2009, Calcified Tissue International.

[5]  T. Cox,et al.  Giant osteoclast formation and long-term oral bisphosphonate therapy. , 2009, The New England journal of medicine.

[6]  H. Frost,et al.  Measurement of bone formation in a 57 year old man by means of tetracyclines. , 1960, Henry Ford Hospital medical bulletin.

[7]  Michael A. Parfitt,et al.  A new method for measuring cancellous bone erosion depth: Application to the cellular mechanisms of bone loss in postmenopausal osteoporosis , 1991, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[8]  R. Recker,et al.  Bone histomorphometry : techniques and interpretation , 1983 .

[9]  R. Recker,et al.  Bone Remodeling Increases Substantially in the Years After Menopause and Remains Increased in Older Osteoporosis Patients , 2004, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[10]  R. Recker,et al.  The label escape error: determination of the active bone-forming surface in histologic sections of bone measured by tetracycline double labels. , 1982, Metabolic bone disease & related research.

[11]  H. Frost,et al.  On the rat model of human osteopenias and osteoporoses. , 1992, Bone and mineral.

[12]  A. Parfitt.  Bone histomorphometry: standardization of nomenclature, symbols and units (summary of proposed system). , 1988, Bone.

[13]  G V Cochran,et al.  A new manual method for assessing two‐dimensional cancellous bone structure: Comparison between iliac crest and lumbar vertebra , 1991, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[14]  R. Erben,et al.  Trabecular and endocortical bone surfaces in the rat: Modeling or remodeling? , 1996, The Anatomical record.

[15]  R. Recker,et al.  Histomorphometric evaluation of daily and intermittent oral ibandronate in women with postmenopausal osteoporosis: results from the BONE study , 2004, Osteoporosis International.

[16]  John P. Bilezikian,et al.  Principles of Bone Biology , 1996 .

[17]  R. Recker.  Should we be concerned about giant osteoclasts , 2009 .

[18]  S. Fihn,et al.  Trimethoprim-sulfamethoxazole for acute dysuria in women: a single-dose or 10-day course. A double-blind, randomized trial. , 1988, Annals of internal medicine.

[19]  R. Recker,et al.  Static and tetracycline‐based bone histomorphometric data from 34 normal postmenopausal females , 1988, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[20]  R. Weinstein,et al.  Apoptosis and osteoporosis. , 2000, The American journal of medicine.

[21]  T. Wronski,et al.  Temporal relationship between bone loss and increased bone turnover in ovariectomized rats , 1988, Calcified Tissue International.

[22]  S. Boonen,et al.  Effects of Intravenous Zoledronic Acid Once Yearly on Bone Remodeling and Bone Structure , 2007, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[23]  C. Hernandez.  How can bone turnover modify bone strength independent of bone mass? , 2008, Bone.

[24]  R R Recker,et al.  A comparison of iliac bone histomorphometric data in post-menopausal osteoporotic and normal subjects. , 1990, Bone and mineral.

[25]  D. Kimmel.  Animal Models in Osteoporosis Research , 2002 .

[26]  H. Weinans,et al.  Mechanical Consequences of Bone Loss in Cancellous Bone , 2001, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[27]  A. Parfitt,et al.  Frequency distributions of tetracycline‐based measurements: Implications for the interpretation of bone formation indices in the absence of double‐labeled surfaces , 1990, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[28]  F. Melsen,et al.  Trabecular bone resorption depth decreases with age: differences between normal males and females. , 1985, Bone.