Glucose determination in human aqueous humor with Raman spectroscopy.

It has been suggested that spectroscopic analysis of the aqueous humor of the eye could be used to indirectly predict blood glucose levels in diabetics noninvasively. We have been investigating this potential using Raman spectroscopy in combination with partial least squares (PLS) analysis. We have determined that glucose at clinically relevant concentrations can be accurately predicted in human aqueous humor in vitro using a PLS model based on artificial aqueous humor. We have further determined that with proper instrument design, the light energy necessary to achieve clinically acceptable prediction of glucose does not damage the retinas of rabbits and can be delivered at powers below internationally acceptable safety limits. Herein we summarize our current results and address our strategies to improve instrument design.

[1]  E. R. Berry,et al.  Ocular spectral characteristics as related to hazards from lasers and other light sources. , 1968, American journal of ophthalmology.

[2]  J. M. Khosrofian,et al.  Measurement of a Gaussian laser beam diameter through the direct inversion of knife-edge data. , 1983, Applied optics.

[3]  John Michael Morookian,et al.  Measurement of aqueous glucose in a model anterior chamber using Raman spectroscopy , 2002 .

[4]  David M. Haaland,et al.  Post-Prandial Blood Glucose Determination by Quantitative Mid-Infrared Spectroscopy , 1992 .

[5]  R. Lasker,et al.  The diabetes control and complications trial. Implications for policy and practice. , 1993, The New England journal of medicine.

[6]  R J McNichols,et al.  Optical glucose sensing in biological fluids: an overview. , 2000, Journal of biomedical optics.

[7]  G L Coté,et al.  Noninvasive and minimally-invasive optical monitoring technologies. , 2001, The Journal of nutrition.

[8]  David E. Booth,et al.  Chemometrics: Data Analysis for the Laboratory and Chemical Plant , 2004, Technometrics.

[9]  J. Soeldner,et al.  Practical Developments in Diabetes Research , 1972, Diabetes.

[10]  Y Wang,et al.  Rapid, noninvasive concentration measurements of aqueous biological analytes by near-infrared Raman spectroscopy. , 1996, Applied optics.

[11]  G. Coté,et al.  Measurement of the glucose transport time delay between the blood and aqueous humor of the eye for the eventual development of a noninvasive glucose sensor. , 2001, Diabetes technology & therapeutics.

[12]  W. T. Ham,et al.  The relative absorption of thermal energy in retina and choroid. , 1962, Investigative ophthalmology.

[13]  D. Cox,et al.  Evaluating Clinical Accuracy of Systems for Self-Monitoring of Blood Glucose , 1987, Diabetes Care.

[14]  G. W. Small,et al.  Multivariate calibration standardization across instruments for the determination of glucose by Fourier transform near-infrared spectrometry. , 2003, Analytical chemistry.

[15]  W. March,et al.  Noninvasive Glucose Monitoring of the Aqueous Humor of the Eye: Part I. Measurement of Very Small Optical Rotations , 1982, Diabetes Care.

[16]  P. Steffes.  Laser-based measurement of glucose in the ocular aqueous humor: an efficacious portal for determination of serum glucose levels. , 1999, Diabetes technology & therapeutics.

[17]  Gerard L. Cot,et al.  Application of a multivariate technique to Raman spectra for quantification of body chemicals , 1995, IEEE Transactions on Biomedical Engineering.

[18]  Massoud Motamedi,et al.  Raman Spectroscopy for Non-Invasive Characterization of Ocular Tissue: Potential for Detection of Biological Molecules , 1997 .

[19]  William T. Ham,et al.  The nature of retinal radiation damage: Dependence on wavelength, power level and exposure time , 1980, Vision Research.

[20]  O. Khalil,et al.  Spectroscopic and clinical aspects of noninvasive glucose measurements. , 1999, Clinical chemistry.

[21]  M. Storrie-Lombardi,et al.  A noninvasive glucose monitor: preliminary results in rabbits. , 1999, Diabetes technology & therapeutics.

[22]  Gary G. Koch,et al.  Categorical Data Analysis Using The SAS1 System , 1995 .

[23]  Henk Spekreijse,et al.  UvA-DARE ( Digital Academic Repository ) Near infrared light absorption in the human eye media , 2022 .

[24]  C. E. Hasty,et al.  Analysis of metabolites in aqueous solutions by using laser Raman spectroscopy. , 1993, Applied optics.

[25]  Gerard L. Coté,et al.  Emerging biomedical sensing technologies and their applications , 2003 .

[26]  F.M. Ham,et al.  Determination of glucose concentrations in an aqueous matrix from NIR spectra using optimal time-domain filtering and partial least-squares regression , 1997, IEEE Transactions on Biomedical Engineering.

[27]  S. Pohjola.  The glucose content of the aqueous humour in man. , 1966, Acta ophthalmologica.

[28]  A. Hughes,et al.  A schematic eye for the rabbit. , 1972, Vision research.