The incorporation of the -amino acid residues into specific positions in the strands and -turn segments of
peptide hairpins is being systematically explored. The presence of an additional torsion variable about the
C()C() bond () enhances the conformational repertoire in -residues. The conformational analysis of
three designed peptide hairpins composed of /-hybrid segments is described: Boc-Leu-Val-Val-DPro-Phe-
Leu-Val-Val-OMe (1), Boc-Leu-Val-Val-DPro-Gly-Leu-Val-Val-OMe (2), and Boc-Leu-Val-Phe-Val-DPro-
Gly-Leu-Phe-Val-Val-OMe (3). 500-MHz 1H-NMR Analysis supports a preponderance of -hairpin
conformation in solution for all three peptides, with critical cross-strand NOEs providing evidence for the
proposed structures. The crystal structure of peptide 2 reveals a -hairpin conformation with two -residues
occupying facing, non-H-bonded positions in antiparallel -strands. Notably, Val(3 ) adopts a gauche
conformation about the C()C() bond (65) without disturbing cross-strand H-bonding. The crystal
structure of 2, together with previously published crystal structures of peptides 3 and Boc-Phe-Phe-DPro-Gly-
Phe-Phe-OMe, provide an opportunity to visualize the packing of peptide sheets with local −polar segments×
formed as a consequence of reversal peptide-bond orientation. The available structural evidence for hairpins
suggests that -residues can be accommodated into nucleating turn segments and into both the H-bonding and
non-H-bonding positions on the strands.