Quantitative microchromatographic determination of hemoglobin f in patients with hemoglobins s and/or c

The improved microchromatographic procedure for the detection of Hb‐S and/or Hb‐C in cord blood at birth (Schroeder et al.: J. Lab Clin Med 86:528‐532, 1975) as well as a modification thereof may also be used for the quantitative determination of Hb‐F in the presence of Hb‐S and/or Hb‐C. However, Hb‐A interferes and must be absent. The methods use 0.5 × 6 cm columns of CM‐cellulose with Tris or Bis‐tris developers and require 2‐4 hr to complete. At low percentages of Hb‐F, the sharper zone of the Tris method is more easily visible than that of the Bis‐tris method, but the latter is a somewhat more rapid procedure.