A unique secreted adenovirus E 3 protein binds to the leukocyte common antigen CD 45 and modulates leukocyte functions

Isoform identification and discovery are an important goal for transcriptome analysis because the majority of human genes express multiple isoforms with contextand tissue-specific functions. Better annotation of isoforms will also benefit downstream analysis such as expression quantification. Current RNA-Seq methods based on short-read sequencing are not reliable for isoform discovery. In this study (pp. E4821–E4830) we developed a new method based on the combined analysis of short reads and long reads generated, respectively, by secondand third-generation sequencing and applied this method to obtain a comprehensive characterization of the transcriptome of the human embryonic stem cell. The results showed that large gain in sensitivity and specificity can be achieved with this strategy.