Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants.

Nonmating mutants were also isolated from haploid strains of yeast of both mating types. The mutants were characterized with respect to their ability to produce and respond to specific yeast sex factors, their ability to mate at low frequencies, and the ability of the low-frequency diploids to sporulate. Loss of the ability to mate by either mating type was invariably accompanied by the loss of one or more, and in some cases, all, of the above capabilities. The results strongly indicate that the sex factors are functionally involved in the conjugation process.

[1]  V. Mackay,et al.  Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of nonmating mutants. , 1974, Genetics.

[2]  E. Bücking-Throm,et al.  Purification and partial characterization of -factor, a mating-type specific inhibitor of cell reproduction from Saccharomyces cerevisiae. , 1973, European journal of biochemistry.

[3]  Y. Oshima,et al.  Mutational nature of an allele-specific conversion of the mating type by the homothallic gene HO alpha in Saccharomyces. , 1970, Genetics.

[4]  V. Mackay,et al.  Saccharomyces cerevisiae: A Diffusible Sex Factor , 1970, Science.

[5]  H. Roman,et al.  The effect of the mating-type alleles on intragenic recombination in yeast. , 1968, Genetics.

[6]  Y. Oshima,et al.  An allele specific and a complementary determinant controlling homothallism in Saccharomyces oviformis. , 1967, Genetics.

[7]  L. Hartwell,et al.  Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast , 1967, Journal of bacteriology.

[8]  T. Takahashi.  Sexuality and its evolution in Saccharomyces. , 1961 .

[9]  T. Takahashi,et al.  Bisexual Mating Reaction in Saccharomyces Chevalieri. , 1959, Genetics.

[10]  J. D. Levi.  Mating Reaction in Yeast , 1956, Nature.

[11]  C C Lindegren,et al.  A New Method for Hybridizing Yeast. , 1943, Proceedings of the National Academy of Sciences of the United States of America.

[12]  L. Hartwell.  Synchronization of haploid yeast cell cycles, a prelude to conjugation. , 1973, Experimental cell research.

[13]  L. J. Wickerham.  A critical evaluation of the nitrogen assimilation tests commonly used in the classification of yeasts. , 1946, Journal of bacteriology.