Capillary Zone Electrophoresis

The contributions of diffusion, Joule heating, sampling and electroosmotic flow to the dispersion of zones separated by capillary zone electrophoresis were evaluated theoretically and verified experimentally. The theoretical model predicts that an optimum electric field strength, E, exists at which the number of theoretical plates, N, is a maximum. Both open and closed capillaries were considered and a simple formula was derived for the calculation of a maximumm with a given experimental arrangement. The theoretical efficiency curves, N vs. E, were calculated and compared with those obtained experimentally in capillaries of various I.D.'s and filled with background electrolytes of various conductivities. The possibilities of minimization of some dispersion factors are discussed.

[1]  M. Sepaniak,et al.  Column efficiency in micellar electrokinetic capillary chromatography , 1987 .

[2]  P. Boček,et al.  High-speed isotachophoresis: current supply and detection system , 1975 .

[3]  James W. Jorgenson,et al.  Zone electrophoresis in open-tubular glass capillaries , 1981 .

[4]  G. Guiochon,et al.  Axial dispersion in open-tubular capillary liquid chromatography with electroosmotic flow , 1984 .

[5]  H. Lauer,et al.  Capillary zone electrophoresis of proteins in untreated fused silica tubing , 1986 .

[6]  T. Nee,et al.  Theory of the electroosmosis effect in the electrophoresis , 1975 .

[7]  F. Everaerts,et al.  High-performance zone electrophoresis , 1979 .

[8]  T. Tsuda,et al.  Separation of nucleotides by high-voltage capillary electrophoresis. , 1983, Journal of applied biochemistry.

[9]  K. Otsuka,et al.  Electrokinetic chromatography with micellar solution and open-tubular capillary , 1985 .

[10]  A. Casoli,et al.  Determination of Uranium by Reversed-Phase High-Performance Liquid Chromatography , 1985 .

[11]  J. Hinckley,et al.  Electrophoretic thermal theory : II. Steady-state radial temperature gradients in circular section columns , 1975 .

[12]  F. Everaerts,et al.  Effect of electroosmosis on detection in isotachophoresis , 1983 .

[13]  S. Hjertén,et al.  Free zone electrophoresis. , 1967, Chromatographic reviews.

[14]  F. Everaerts,et al.  Concentration distributions in free zone electrophoresis , 1979 .

[15]  J. Hessels,et al.  On the efficiency of gel electrophoresis , 1972 .

[16]  M. P. Maskarinec,et al.  Separation of modified nucleic acid constituents by micellar electrokinetic capillary chromatography. , 1987, Journal of chromatography.

[17]  John L. Anderson,et al.  ELECTROOSMOSIS THROUGH PORES WITH NONUNIFORMLY CHARGED WALLS , 1985 .