Effect of low-level α-radiation on bioluminescent assay systems of various complexity

This study addresses the effects of low-level α-radiation on bioluminescent assay systems of different levels of organization: in vivo and in vitro. Three bioluminescent assay systems are used: intact bacteria, lyophilized bacteria, and bioluminescent system of coupled enzyme reactions. Solutions of^241Am(NO_3)_3 are used as a source of α-radiation. It has been shown that activation processes predominate in all the three bioluminescent assay systems subjected to short-term exposure (20–55 h) and inhibition processes in the systems subjected to longer-term exposure to radiation. It has been found that these effects are caused by the radiation component of^241Am^3+ impact. The intensity of the^241Am^3+ effect on the bioluminescent assay systems has been shown to depend on the^241Am^3+ concentration, level of organization and integrity of the bioluminescent assay system. The bioluminescent assay systems in vivo have been found to be highly sensitive to^241Am^3+ (up to 10^−17 M).

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