Semiautomatic and rapid quantification of heartbeat parameters in Drosophila using optical coherence tomography imaging

Abstract. We report a semiautomatic algorithm that is specialized for rapid analysis of beat-to-beat contraction-relaxation parameters of the heart in Drosophila. The presented algorithm adapts the general graph theoretical image segmentation algorithm and a histogram-based thresholding algorithm, which can measure many cardiac parameters, including heart rate, heart period, diastolic and systolic intervals, and end-diastolic and end-systolic areas. Additionally, dynamic cardiac functions, such as arrhythmia index and percent fractional shortening, can be automatically calculated for all the recorded heartbeats over significant periods of time.

[1]  John Shawe-Taylor,et al.  Canonical Correlation Analysis: An Overview with Application to Learning Methods , 2004, Neural Computation.

[2]  T. W. Ridler,et al.  Picture thresholding using an iterative selection method. , 1978 .

[3]  Joseph A. Izatt,et al.  In Vivo Imaging of the Adult Drosophila melanogaster Heart With Real-Time Optical Coherence Tomography , 2006 .

[4]  Karen Ocorr,et al.  Semi-automated Optical Heartbeat Analysis of small hearts. , 2009, Journal of visualized experiments : JoVE.

[5]  Laurent Perrin,et al.  Steroid-dependent modification of Hox function drives myocyte reprogramming in the Drosophila heart , 2005, Development.

[6]  Leo Grady,et al.  Random Walks for Image Segmentation , 2006, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[7]  Rui Seara,et al.  Image segmentation by histogram thresholding using fuzzy sets , 2002, IEEE Trans. Image Process..

[8]  Adrian Bradu,et al.  Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart. , 2009, Journal of biophotonics.

[9]  John S. Strobel,et al.  Nonpharmacologic Validation of the Intrinsic Heart Rate in Cardiac Transplant Recipients , 1999, Journal of Interventional Cardiac Electrophysiology.

[10]  Joseph A Izatt,et al.  Drosophila as a model for the identification of genes causing adult human heart disease , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Karen Ocorr,et al.  KCNQ potassium channel mutations cause cardiac arrhythmias in Drosophila that mimic the effects of aging , 2007, Proceedings of the National Academy of Sciences.

[12]  A. McCulloch,et al.  Age-Associated Cardiac Dysfunction in Drosophila melanogaster , 2001, Circulation research.

[13]  R. Bodmer,et al.  Insulin regulation of heart function in aging fruit flies , 2004, Nature Genetics.

[14]  Adrian Bradu,et al.  Arrhythmia Caused by a Drosophila Tropomyosin Mutation Is Revealed Using a Novel Optical Coherence Tomography Instrument , 2010, PloS one.

[15]  Gareth Funka-Lea,et al.  Multi-label Image Segmentation for Medical Applications Based on Graph-Theoretic Electrical Potentials , 2004, ECCV Workshops CVAMIA and MMBIA.

[16]  K. Slăma,et al.  A new look at the comparative physiology of insect and human hearts. , 2012, Journal of insect physiology.

[17]  Marie-Pierre Jolly,et al.  Interactive graph cuts for optimal boundary & region segmentation of objects in N-D images , 2001, Proceedings Eighth IEEE International Conference on Computer Vision. ICCV 2001.

[18]  Benjamin J Vakoc,et al.  Heart wall velocimetry and exogenous contrast-based cardiac flow imaging in Drosophila melanogaster using Doppler optical coherence tomography. , 2010, Journal of biomedical optics.

[19]  Willard L. Miranker,et al.  Fast Hybrid Solution of Algebraic Systems , 1990, SIAM J. Sci. Comput..

[20]  Rita Cucchiara,et al.  A semi-automatic system for segmentation of cardiac M-mode images , 2006, Pattern Analysis and Applications.

[21]  Rolf Bodmer,et al.  Screening assays for heart function mutants in Drosophila. , 2004, BioTechniques.

[22]  Karen Ocorr,et al.  A new method for detection and quantification of heartbeat parameters in Drosophila, zebrafish, and embryonic mouse hearts. , 2009, BioTechniques.

[23]  Karen Ocorr,et al.  Myosin transducer mutations differentially affect motor function, myofibril structure, and the performance of skeletal and cardiac muscles. , 2007, Molecular biology of the cell.

[24]  Anthony J. Yezzi,et al.  Anti-geometric diffusion for adaptive thresholding and fast segmentation , 2003, IEEE Trans. Image Process..

[25]  James A. Sethian,et al.  Level Set Methods and Fast Marching Methods , 1999 .

[26]  Meng-Tsan Tsai,et al.  Observations of cardiac beating behaviors of wild‐type and mutant Drosophilae with optical coherence tomography , 2011, Journal of biophotonics.

[27]  Karen Ocorr,et al.  The ATP-sensitive potassium (KATP) channel-encoded dSUR gene is required for Drosophila heart function and is regulated by tinman , 2006, Proceedings of the National Academy of Sciences.

[28]  J. Gardin,et al.  Prevalence of atrial fibrillation in elderly subjects (the Cardiovascular Health Study). , 1994, The American journal of cardiology.

[29]  Gaurav Kaushik,et al.  In situ mechanical analysis of myofibrillar perturbation and aging on soft, bilayered Drosophila myocardium. , 2011, Biophysical journal.

[30]  R. Levine,et al.  Glutamatergic Innervation of the Heart Initiates Retrograde Contractions in Adult Drosophila melanogaster , 2005, The Journal of Neuroscience.

[31]  Linda G. Shapiro,et al.  Image Segmentation Techniques , 1984, Other Conferences.

[32]  T Opthof,et al.  The normal range and determinants of the intrinsic heart rate in man. , 2000, Cardiovascular research.