Antibodies to HMG proteins in patients with drug-induced autoimmunity.

OBJECTIVE To determine the prevalence of autoantibodies to high-mobility group (HMG) proteins in sera from patients with drug-induced lupus (DIL). METHODS Forty-two patients who developed autoantibodies and/or lupus after treatment with procainamide or other drugs were tested for HMG autoantibodies by immunoblotting and enzyme-linked immunosorbent assay (ELISA). RESULTS Twenty-eight of the 42 sera (67%) bound HMG-14 and/or HMG-17. In comparison, 9 of 42 (21%) bound HMG-1 and/or HMG-2. There was a good correlation between ELISA results and binding on immunoblots. CONCLUSION The high prevalence of antibodies to the nucleosomal core HMGs (HMG-14 and HMG-17) in DIL patients adds evidence implicating nucleosomes as immunogens in drug-induced autoimmunity.

[1]  L. Chabanne,et al.  Canine Systemic Lupus Erythematosus. II: Antinuclear Antibodies , 1992 .

[2]  M. Salazar-Páramo,et al.  Systemic lupus erythematosus induced by isoniazid. , 1992, Annals of the rheumatic diseases.

[3]  R. Burlingame,et al.  Autoantibodies associated with lupus induced by diverse drugs target a similar epitope in the (H2A-H2B)-DNA complex. , 1992, The Journal of clinical investigation.

[4]  M. Wener,et al.  Drug induced systemic lupus erythematosus due to ophthalmic timolol. , 1992, The Journal of rheumatology.

[5]  M. Bustin,et al.  Autoantibodies to the chromosomal protein HMG-17 in juvenile rheumatoid arthritis. , 1992, Arthritis and rheumatism.

[6]  R. Burlingame,et al.  Drug-induced anti-histone autoantibodies display two patterns of reactivity with substructures of chromatin. , 1991, The Journal of clinical investigation.

[7]  S. West,et al.  D-penicillamine-induced lupus erythematosus. , 1990, Arthritis and rheumatism.

[8]  H. Truckenbrodt,et al.  Autoantibodies to nonhistone chromosomal proteins HMG-1 and HMG-2 in sera of patients with juvenile rheumatoid arthritis. , 1990, Arthritis and rheumatism.

[9]  P. J. González,et al.  Structural and transcriptional properties of different nucleosomal particles containing high mobility group proteins 14 and 17 (HMG 14/17). , 1990, The Journal of biological chemistry.

[10]  S. West,et al.  Methyldopa-induced systemic lupus erythematosus. , 1989, Arthritis and rheumatism.

[11]  P. Cary,et al.  Antibodies from patients with drug-induced and idiopathic lupus erythematosus react with epitopes restricted to the amino and carboxyl termini of histone. , 1985, Journal of immunology.

[12]  M. Bustin,et al.  Autoantibodies to nucleosomal proteins: antibodies to HMG-17 in autoimmune diseases. , 1982, Science.