Estimation of viable cell count after fluorescein diacetate staining using phosphorimager analysis.
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1.Barany, F. 1985. Single-stranded hexameric linkers: a system for in-phase insertion mutagenesis and protein engineering. Gene 37:111-123. 2.Barany, F. 1985. Two-codon insertion mutagenesis of plasmid genes by using singlestranded hexameric oligonucleotides. Proc. Natl. Acad. Sci. USA 82:4202-4206. 3.Botstein, D. and D. Shortle. 1985. Strategies and applications of in vitro mutagenesis. Science 229:1193-1201. 4.Erlich, H.A. (Ed.). 1989. PCR Technology: Principles and Applications for DNA Amplification. Stockton Press, New York. 5.Heffron, F., M. So and B.J. McCarthy. 1978. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc. Natl. Acad. Sci. USA 75:6012-6016. 6.Henikoff, S. 1987. Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods Enzymol. 155:156-165. 7.Riedel, H., L. Su and H. Hansen. 1993. Yeast phenotype classifies mammalian protein kinase C cDNA mutants. Mol. Cell Biol. 13:4728-4735. 8.Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY. 9.Smith, M. 1985. In vitro mutagenesis. Annu. Rev. Genet. 19:423-462. 10.Zoller, M.J. 1991. New molecular biology methods for protein engineering. Curr. Opin. Biotechnol. 2:526-531.