An ISFET biosensor for the monitoring of maltose‐induced conformational changes in MBP

Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge‐dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate‐specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose‐induced structural alterations in MBP.

[1]  P. Woias,et al.  Protein detection with a novel ISFET-based zeta potential analyzer. , 1999, Biosensors & bioelectronics.

[2]  Marcus Fehr,et al.  Visualization of maltose uptake in living yeast cells by fluorescent nanosensors , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[3]  B Wolf,et al.  Monitoring of cellular signalling and metabolism with modular sensor-technique: the PhysioControl-Microsystem (PCM). , 1998, Biosensors & bioelectronics.

[4]  Jin Kyeong Kim,et al.  Direct immobilization of protein g variants with various numbers of cysteine residues on a gold surface. , 2007, Analytical chemistry.

[5]  Itamar Willner,et al.  Probing antigen-antibody binding processes by impedance measurements on ion-sensitive field-effect transistor devices and complementary surface plasmon resonance analyses: development of cholera toxin sensors. , 2002, Analytical chemistry.

[6]  Igor L. Medintz,et al.  Maltose-binding protein: a versatile platform for prototyping biosensing. , 2006, Current opinion in biotechnology.

[7]  Nguyen-Huu Xuong,et al.  Crystal Structure of a Complex Between the Catalytic and Regulatory (RIα) Subunits of PKA , 2005, Science.

[8]  Chen Jianrong,et al.  Ion sensitive field effect transducer-based biosensors. , 2003, Biotechnology advances.

[9]  Fred J Sigworth,et al.  Importance of the Debye screening length on nanowire field effect transistor sensors. , 2007, Nano letters.

[10]  N. Jaffrezic‐Renault,et al.  Enzyme biosensors based on ion-selective field-effect transistors. , 2006, Analytica chimica acta.

[11]  M. Zourob,et al.  An integrated metal clad leaky waveguide sensor for detection of bacteria. , 2005, Analytical chemistry.

[12]  S D Moss,et al.  Chemically sensitive field-effect transistors. , 1976, Biomedical engineering.

[13]  A. Means,et al.  Calmodulin: a prototypical calcium sensor. , 2000, Trends in cell biology.

[14]  M. Lemmon.  Faculty Opinions recommendation of Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA. , 2005 .

[15]  W. Frommer,et al.  Minimally invasive dynamic imaging of ions and metabolites in living cells. , 2004, Current opinion in plant biology.

[16]  Teruyuki Nishimura,et al.  Structural basis for allosteric regulation of the monomeric allosteric enzyme human glucokinase. , 2004, Structure.

[17]  Jinyoung Jeong,et al.  Monitoring of conformational change in maltose binding protein using split green fluorescent protein. , 2006, Biochemical and biophysical research communications.

[18]  I. Willner,et al.  An integrated NAD+-dependent enzyme-functionalized field-effect transistor (ENFET) system: development of a lactate biosensor. , 2000, Biosensors & bioelectronics.

[19]  P Bergveld,et al.  Development of an ion-sensitive solid-state device for neurophysiological measurements. , 1970, IEEE transactions on bio-medical engineering.

[20]  P Bergveld,et al.  A new approach to immunoFET operation. , 1990, Biosensors & bioelectronics.

[21]  P. Fromherz,et al.  A neuron-silicon junction: a Retzius cell of the leech on an insulated-gate field-effect transistor. , 1991, Science.

[22]  Jang-Kyoo Shin,et al.  An FET-type charge sensor for highly sensitive detection of DNA sequence. , 2004, Biosensors & bioelectronics.

[23]  P. Sorger,et al.  Electronic detection of DNA by its intrinsic molecular charge , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[24]  M. Schöning,et al.  Recent advances in biologically sensitive field-effect transistors (BioFETs). , 2002, The Analyst.