论文信息 - Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals.

Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals.

Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.

[1] G. Petsko. Preparation of isomorphous heavy-atom derivatives. , 1985, Methods in enzymology.

[2] G. Petsko,et al. The 1.20 A resolution crystal structure of the aminopeptidase from Aeromonas proteolytica complexed with tris: a tale of buffer inhibition. , 2002, Structure.

[3] G A Petsko,et al. Protein crystallography at sub-zero temperatures: cryo-protective mother liquors for protein crystals. , 1975, Journal of molecular biology.

[4] G. G. Stokes. "J." , 1890, The New Yale Book of Quotations.

[5] K. Y. Choi,et al. Identification of active site residues by site-directed mutagenesis of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B , 1995, Journal of bacteriology.

[6] A. McPherson. A comparison of salts for the crystallization of macromolecules , 2001, Protein science : a publication of the Protein Society.

[7] Gregory A Petsko,et al. 2.4 A resolution crystal structure of the prototypical hormone-processing protease Kex2 in complex with an Ala-Lys-Arg boronic acid inhibitor. , 2003, Biochemistry.