An All Optical Microfluidic Sorter

We present a microfluidic cell sorter that is able to count, characterize and sort micrometer sized particles and cells. In addition to optical counting and characterization, also sorting is performed by optical forces. The device is optimized for simplicity. The microfluidic channels and optical waveguides that carry the illuminating, detecting and sorting light form a single integrated structure, all built from the same material in a single photopolymerization step.

[1]  Elinore M Mercer,et al.  Microfluidic sorting of mammalian cells by optical force switching , 2005, Nature Biotechnology.

[2]  A. Givan,et al.  Flow Cytometry: First Principles , 1992 .

[3]  Alan P. Morrison,et al.  Development of a microfluidic device for fluorescence activated cell sorting , 2002 .

[4]  K. Dholakia,et al.  Microfluidic sorting in an optical lattice , 2003, Nature.

[5]  T. Nyström,et al.  A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes. , 2007, Lab on a chip.

[6]  Martin Siler,et al.  Optical sorting and detection of submicrometer objects in a motional standing wave , 2006 .

[7]  J. Kutter,et al.  Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter. , 2003, Lab on a chip.

[8]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[9]  R. Hertzberg,et al.  High-throughput screening: new technology for the 21st century. , 2000, Current opinion in chemical biology.

[10]  J M Cooper,et al.  High-throughput screens for postgenomics: studies of protein crystallization using microsystems technology. , 2002, Analytical chemistry.

[11]  J.P. Kutter,et al.  Microchip flow cytometer with integrated polymer optical elements for measurement of scattered light , 2004, 17th IEEE International Conference on Micro Electro Mechanical Systems. Maastricht MEMS 2004 Technical Digest.

[12]  J. Squier,et al.  Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping. , 2006, Lab on a chip.

[13]  Olav Solgaard,et al.  Design and operation of a microfluidic sorter for Drosophila embryos , 2004 .

[14]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.