Comparison of Eight Methods To Detect Vancomycin Resistance in Enterococci

ABSTRACT A collection of genetically unrelated vancomycin-resistant enterococci (VRE) including 50 vanA, 15 vanB, 50 vanC1, and 30 vanC2 VRE were used to evaluate the accuracy of eight currently available susceptibility test methods (agar dilution, disk diffusion, E-test, agar screen plate, Vitek GPS-TA and GPS-101, and MicroScan overnight and rapid panels).vanA VRE were detected by all methods. vanB VRE were often not detected by Vitek GPS-TA and MicroScan rapid (sensitivities, 47 and 53%, respectively), though the new Vitek GPS-101 was found to be a significant improvement. E-test and the agar screen were the only two methods detecting all VRE, including thevanC1/C2 VRE.

[1]  M. Ferraro,et al.  New vancomycin disk diffusion breakpoints for enterococci. The National Committee for Clinical Laboratory Standards Working Group on Enterococci , 1992, Journal of clinical microbiology.

[2]  F. Tenover,et al.  Ability of commercial and reference antimicrobial susceptibility testing methods to detect vancomycin resistance in enterococci , 1995, Journal of clinical microbiology.

[3]  P. Courvalin,et al.  Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR , 1995, Journal of clinical microbiology.

[4]  R. Leclercq,et al.  Resistance to glycopeptides in enterococci. , 1997, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[5]  D. Sahm,et al.  Reliability of the E test for detection of ampicillin, vancomycin, and high-level aminoglycoside resistance in Enterococcus spp , 1993, Journal of clinical microbiology.

[6]  D E Low,et al.  Detection of vancomycin resistance in Enterococcus species , 1992, Journal of clinical microbiology.

[7]  J. Waitz.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically , 1990 .

[8]  Hospital Infection Control Practices Advisory Committee (HICPAC) , 1995 .

[9]  L. Gutmann,et al.  Methicillin-resistant Staphylococus aureus and vancomycin-resistant enterococci: therapeutic realities and possibilities , 1997, The Lancet.

[10]  J. Duval,et al.  Vancomycin resistance gene vanC is specific to Enterococcus gallinarum , 1992, Antimicrobial Agents and Chemotherapy.

[11]  J. Tokars,et al.  Ability of clinical laboratories to detect antimicrobial agent-resistant enterococci , 1993 .

[12]  Nosocomial enterococci resistant to vancomycin--United States, 1989-1993. , 1993, MMWR. Morbidity and mortality weekly report.

[13]  Nicole,et al.  Fecal carriage of vancomycin-resistant enterococci in hospitalized patients and those living in the community in The Netherlands , 1997, Journal of clinical microbiology.

[14]  B. Murray.  Editorial Response: What Can We Do About Vancomycin-Resistant Enterococci? , 1995 .

[15]  D. Sahm,et al.  In vitro detection of enterococcal vancomycin resistance , 1990, Antimicrobial Agents and Chemotherapy.

[16]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.

[17]  Elaine Larson,et al.  Recommendations for preventing the spread of vancomycin resistance. Recommendations of the Hospital Infection Control Practices Advisory Committee (HICPAC). , 1995, MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports.

[18]  M. Weinstein,et al.  Development of a standardized screening method for detection of vancomycin-resistant enterococci , 1994, Journal of clinical microbiology.

[19]  C. Thornsberry,et al.  Problems with the disk diffusion test for detection of vancomycin resistance in enterococci , 1989, Journal of clinical microbiology.