Non-Destructive Analysis of the Internal Anatomical Structures of Mosquito Specimens Using Optical Coherence Tomography

The study of mosquitoes and analysis of their behavior are of crucial importance in the on-going efforts to control the alarming increase in mosquito-borne diseases. Furthermore, a non-destructive and real-time imaging technique to study the anatomical features of mosquito specimens can greatly aid the study of mosquitoes. In this study, we demonstrate the three-dimensional imaging capabilities of optical coherence tomography (OCT) for structural analysis of Anopheles sinensis mosquitoes. The anatomical features of An. sinensis head, thorax, and abdominal regions, along with the morphology of internal structures, such as foregut, midgut, and hindgut, were studied using OCT imaging. Two-dimensional and three-dimensional OCT images, used in conjunction with histological images, proved useful for anatomical analysis of mosquito specimens. By presenting this work as an initial study, we demonstrate the applicability of OCT for future mosquito-related entomological research, and also in identifying changes in mosquito anatomical structure.

[1]  A. Tatem,et al.  The Limits and Intensity of Plasmodium falciparum Transmission : Implications for Malaria Control and Elimination Worldwide , 2007 .

[2]  Beop-Min Kim,et al.  Optically deviated focusing method based high-speed SD-OCT for in vivo retinal clinical applications , 2016 .

[3]  W. Choochote,et al.  Multiplex assay to identify Korean vectors of malaria , 2010, Molecular ecology resources.

[4]  Jeehyun Kim,et al.  Dual-path handheld system for cornea and retina imaging using optical coherence tomography , 2017 .

[5]  Pil Un Kim,et al.  Fast Industrial Inspection of Optical Thin Film Using Optical Coherence Tomography , 2016, Sensors.

[6]  Jeehyun Kim,et al.  Bio-Photonic Detection and Quantitative Evaluation Method for the Progression of Dental Caries Using Optical Frequency-Domain Imaging Method , 2016, Sensors.

[7]  Michelle Harvey,et al.  Optical coherence tomography: age estimation of Calliphora vicina pupae in vivo? , 2014, Forensic science international.

[8]  Adrian Bradu,et al.  Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart. , 2009, Journal of biophotonics.

[9]  D. Vaughn,et al.  Impact of dengue virus infection on feeding behavior of Aedes aegypti. , 1997, The American journal of tropical medicine and hygiene.

[10]  R. Wijesinghe,et al.  In vivo observation of metamorphosis of Plodia interpunctella Hübner using three‐dimensional optical coherence tomography , 2017 .

[11]  W. Jiraungkoorskul.  Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva , 2015 .

[12]  Jeehyun Kim,et al.  Optical Coherence Tomography for the Diagnosis and Evaluation of Human Otitis Media , 2015, Journal of Korean medical science.

[13]  G. League,et al.  Comparative structural and functional analysis of the larval and adult dorsal vessel and its role in hemolymph circulation in the mosquito Anopheles gambiae , 2015, Journal of Experimental Biology.

[14]  B. A. Harrison,et al.  Host-Feeding Habits of Culex and Other Mosquitoes (Diptera: Culicidae) in the Borough of Queens in New York City, with Characters and Techniques for Identification of Culex Mosquitoes , 2002, Journal of medical entomology.

[15]  G. Ripandelli,et al.  Optical coherence tomography. , 1998, Seminars in ophthalmology.

[16]  Stephen A. Boppart,et al.  Imaging developing neural morphology using optical coherence tomography , 1996, Journal of Neuroscience Methods.

[17]  Michael A Tolle,et al.  Mosquito-borne diseases. , 2009, Current problems in pediatric and adolescent health care.

[18]  D Maulik,et al.  Zika virus and pregnancy , 2017, The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians.

[19]  W. Crans,et al.  A classification system for mosquito life cycles: life cycle types for mosquitoes of the northeastern United States. , 2004, Journal of vector ecology : journal of the Society for Vector Ecology.

[20]  Young-Ran Ha,et al.  Comparison of the functional features of the pump organs of Anopheles sinensis and Aedes togoi , 2015, Scientific Reports.

[21]  H. Iwamoto.  X-ray diffraction pattern from the flight muscle of Toxorhynchites towadensis reveals the specific phylogenic position of mosquito among Diptera , 2015, Zoological Letters.

[22]  Jeehyun Kim,et al.  Depth enhancement in spectral domain optical coherence tomography using bidirectional imaging modality with a single spectrometer , 2016, Journal of biomedical optics.

[23]  J. Boorman.  Observations on the feeding habits of the mosquito Aedes (Stegomyia) aegypti (Linnaeus): the loss of fluid after a blood-meal and the amount of blood taken during feeding. , 1960, Annals of tropical medicine and parasitology.

[24]  H. Zeller,et al.  West Nile: worldwide current situation in animals and humans. , 2004, Comparative immunology, microbiology and infectious diseases.

[25]  M. K. Kim,et al.  Tomographic three-dimensional imaging of a biological specimen using wavelength-scanning digital interference holography. , 2000, Optics express.

[26]  Jeehyun Kim,et al.  In Vivo Monitoring on Growth and Spread of Gray Leaf Spot Disease in Capsicum annuum Leaf Using Spectral Domain Optical Coherence Tomography , 2016 .

[27]  J. Ribeiro,et al.  Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia , 2009, PLoS neglected tropical diseases.

[28]  Adrian Bradu,et al.  Arrhythmia Caused by a Drosophila Tropomyosin Mutation Is Revealed Using a Novel Optical Coherence Tomography Instrument , 2010, PloS one.