In order to obtain transgenic cabbage lines with insect-resistance,cry1Ia8 gene was transferred to cabbage(Brassica oleracea L.var.capitata L.) with the transformation of hypocotyl segments and cotyledon with petiole via Agrobacterium tumefaciens.Thirty-eight transformants with kanamycin-resistance were obtained.PCR and Southern blot analysis showed that cry1Ia8 gene was integrated into the genome of cabbage.Moreover,RT-PCR and Western blot detection demonstrated that cry1Ia8 gene was expressed in RNA and protein levels.The results of bioassay with the susceptible and resistant diamondback moth(DBM,Plutella xylostella) displayed that most of the transgenic plants were resistant to the larvae of susceptible DBM and also to the insect strain which is resistant to Cry1Ac toxin.