Phenol biosensor based on Sonogel-Carbon transducer with tyrosinase alumina sol-gel immobilization.

A new biosensor for detection of phenols, based on tyrosinase immobilization with alumina sol-gel on Sonogel-Carbon transducer, has been developed. The electrode was prepared using high energy ultrasounds directly applied to the precursors. The alumina sol-gel provided a microenvironment for retaining the native structure and activity of the entrapped enzyme and a very low mass transport barrier to the enzyme substrates. Phenols are oxidized by tyrosinase biosensor to form a detectable product, which was determined at -300 mV vs. Ag/AgCl reference electrode. For phenol, the sensor exhibited a fast response which resulted from the porous structure and high enzyme loading of the sol-gel matrix. The linear range was from 5 x 10(-7)M to 3 x 10(-5)M, with a detection limit of 3 x 10(-7)M. The stability of the biosensor was also evaluated.

[1]  S. Cosnier,et al.  A comparison of amperometric screen-printed, carbon electrodes and their application to the analysis of phenolic compounds present in beers. , 2001, Talanta.

[2]  C. Toh,et al.  Recent advances in analytical chemistry--a material approach. , 2006, Analytica chimica acta.

[3]  K. Temsamani,et al.  The Sonogel-Carbon materials as basis for development of enzyme biosensors for phenols and polyphenols monitoring: a detailed comparative study of three immobilization matrixes. , 2007, Biosensors & bioelectronics.

[4]  B. Ballarin,et al.  Synthesis and electrochemical characterisation of novel sonogel-carbon-polythiophene microstructured electrodes , 2003 .

[5]  Joseph Wang,et al.  Sol–gel materials for electrochemical biosensors , 1999 .

[6]  Huangxian Ju,et al.  Mediator-free phenol sensor based on titania sol-gel encapsulation matrix for immobilization of tyrosinase by a vapor deposition method. , 2003, Biosensors & bioelectronics.

[7]  J. Kong,et al.  Probing trace phenols based on mediator-free alumina sol--gel-derived tyrosinase biosensor. , 2000, Analytical chemistry.

[8]  Ovadia Lev,et al.  Sol-Gel Derived Composite Ceramic Carbon Electrodes , 2001 .

[9]  D. Tonelli,et al.  Electrocatalytic activity of cobalt phthalocyanine stabilized by different matrixes , 2002, Analytical and bioanalytical chemistry.

[10]  E. Blanco,et al.  The Sonogel-Carbon electrode as a sol-gel graphite-based electrode. , 2002, Analytical chemistry.

[11]  J. Pawliszyn,et al.  Solid Phase Microextraction for Determining the Distribution of Chemicals in Aqueous Matrices , 1997 .

[12]  J. Wang,et al.  Amperometric biosensor for phenols based on a tyrosinase-graphite-epoxy biocomposite. , 1994, The Analyst.

[13]  John D. Brennan,et al.  Properties and applications of proteins encapsulated within sol–gel derived materials , 2002 .

[14]  S. Cosnier,et al.  A new strategy for the construction of a tyrosinase-based amperometric phenol and o-diphenol sensor , 1993 .

[15]  David J. Best,et al.  THE DETERMINATION OF P-CRESOL IN CHLOROFORM WITH AN ENZYME ELECTRODE USED IN THE ORGANIC-PHASE , 1988 .

[16]  O. Lev,et al.  Sol-Gel-Derived Ceramic-Carbon Composite Electrodes: Introduction and Scope of Applications , 1994 .

[17]  Klaus-Dieter Vorlop,et al.  Methylphenazonium-modified enzyme sensor based on polymer thick films for subnanomolar detection of phenols , 1995 .