Paclitaxel liposomes exert radio-sensitization effect on breast cancer cell line SK-BR-3 by regulating expressions of apoptotic proteins

Purpose: To study the radio-sanitization effect of paclitaxel liposomes on breast cancer cells, SK-BR-3.Methods: Breast cancer cell line SK-BR-3 was cultured and made into a cell suspension. Four groups of cells were used: control and radiotherapy groups, paclitaxel group, and paclitaxel liposome + radiotherapy group (combination group). The growth inhibitory effects of the different treatments on SKBR-3 cells were determined with CCK-8 method. Apoptosis in each group was evaluated with flow cytometry, while Western blotting was employed to assay Bcl-2, Caspase-3 and Bax protein levels.Results: There were marked inhibitory effect on growth of SK-BR-3 cells in drug, radiotherapy and combination groups, relative to control, while apoptosis was greater in combination group than in drug and radiotherapy groups (p < 0.05). The Bcl-2 protein level was higher in radiotherapy, drug and combination groups than in control group, while Caspase-3 and Bax proteins were markedly higher thancontrol values (p < 0.05).Conclusion: Paclitaxel liposomes exert radio-sensitization effect on SK-BR-3 by regulating the levels of apoptotic proteins. This provides a basis for research and development of new drugs. Keywords: Paclitaxel liposomes, Apoptotic proteins, Breast cancer, SK-BR-3, Radiotherapy, Sensitization, Bcl-2, Caspase-3, Bax

[1]  S. Feigenberg,et al.  Optimal Target Delineation and Treatment Techniques in the Era of Conformal Photon and Proton Breast and Regional Nodal Irradiation: Breast Nodal Optimal Target Delineation. , 2019, Practical radiation oncology.

[2]  F. Jiang,et al.  Nocturnal enuresis in obese children: a nation-wide epidemiological study from China , 2019, Scientific Reports.

[3]  M. Jimenez-Del-Rio,et al.  TPEN Exerts Antitumor Efficacy in Murine Mammary Adenocarcinoma Through an H2O2 Signaling Mechanism Dependent on Caspase-3. , 2019, Anti-cancer agents in medicinal chemistry.

[4]  Michael L. Gatza,et al.  Exosomal miRNA confers chemo resistance via targeting Cav1/p-gp/M2-type macrophage axis in ovarian cancer , 2018, EBioMedicine.

[5]  L. You,et al.  Mechanical regulation of breast cancer migration and apoptosis via direct and indirect osteocyte signaling , 2018, Journal of cellular biochemistry.

[6]  Qingli Zhu,et al.  Angiogenesis Research in Mouse Mammary Cancer Based on Contrast-enhanced Ultrasonography: Exploratory Study. , 2018, Academic radiology.

[7]  D. Zylis,et al.  Biweekly Gemcitabine/Nab-Paclitaxel as First-line Treatment for Advanced Pancreatic Cancer. , 2018, In vivo.

[8]  Yue Wang,et al.  Dissecting the role of the CRMP2–neurofibromin complex on pain behaviors , 2017, Pain.

[9]  Thomas E Rohan,et al.  Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism , 2017, Science Translational Medicine.

[10]  S. Johnston,et al.  Abstract 660: Pre-symptomatic detection and early treatment of mammary cancer with anti-PD-L1 in a mouse mode , 2017 .

[11]  Chi-Tang Ho,et al.  Garcinol sensitizes breast cancer cells to Taxol through the suppression of caspase-3/iPLA2 and NF-κB/Twist1 signaling pathways in a mouse 4T1 breast tumor model. , 2017, Food & function.

[12]  M. Kheirkhah,et al.  Effects of Swedish Massage on the Improvement of Mood Disorders in Women with Breast Cancer undergoing Radiotherapy , 2016, Iranian Red Crescent medical journal.

[13]  J. Sparano,et al.  Abstract 244: Paclitaxel increases the assembly and function of the tumor microenvironment of metastasis in breast cancer , 2016 .

[14]  Dinuo Li,et al.  Oxymatrine mediates Bax and Bcl-2 expression in human breast cancer MCF-7 cells. , 2016, Die Pharmazie.