Targeted resequencing analysis of 25 genes commonly mutated in myeloid disorders in del(5q) MDS

Targeted re-sequencing We designed a TruSeq Custom Amplicon panel (TSCA, Illumina), targeting 25 genes mutated in various myeloid malignancies (Table 2). The panel was developed using the online DesignStudio pipeline (http://designstudio.illumina.com, Illumina), and covers a total of 46,604bp with 322 amplicons. In genes with well-defined mutational hotspots only these regions were targeted; otherwise the entire coding sequence of the gene was sequenced.