Laser-induced fluorescent spectroscopy of steroid hormones

The laser-induced fluorescence spectra of steroid hormones - estradiol, estriol, estrone, androstenedione - are obtained at excitation of the fourth harmonic of Nd:YAG laser radiation. The quantum yields of fluorescence of these substances were rated by means of the relative method. They are 1.11 X 10-1, 5.20 X 10-3, 8.47 X 10-5. The water solution of tryptophan was used as a standard. The set-up sensitivity for high and average quantum yields substances has been defined.