Bioelectrochemical enhancement of anaerobic methanogenesis for high organic load rate wastewater treatment in a up-flow anaerobic sludge blanket (UASB) reactor

A coupling process of anaerobic methanogenesis and electromethanogenesis was proposed to treat high organic load rate (OLR) wastewater. During the start-up stage, acetate removal efficiency of the electric-biological reactor (R1) reached the maximization about 19 percentage points higher than that of the control anaerobic reactor without electrodes (R2), and CH4 production rate of R1 also increased about 24.9% at the same time, while additional electric input was 1/1.17 of the extra obtained energy from methane. Coulombic efficiency and current recorded showed that anodic oxidation contributed a dominant part in degrading acetate when the metabolism of methanogens was low during the start-up stage. Along with prolonging operating time, aceticlastic methanogenesis gradually replaced anodic oxidation to become the main pathway of degrading acetate. When the methanogens were inhibited under the acidic conditions, anodic oxidation began to become the main pathway of acetate decomposition again, which ensured the reactor to maintain a stable performance. FISH analysis confirmed that the electric field imposed could enrich the H2/H+-utilizing methanogens around the cathode to help for reducing the acidity. This study demonstrated that an anaerobic digester with a pair of electrodes inserted to form a coupling system could enhance methanogenesis and reduce adverse impacts.

[1]  J. M. Owens,et al.  Renewable methane from anaerobic digestion of biomass , 1997 .

[2]  K. Sasaki,et al.  The membraneless bioelectrochemical reactor stimulates hydrogen fermentation by inhibiting methanogenic archaea , 2013, Applied Microbiology and Biotechnology.

[3]  K. Timmis,et al.  Shift from Acetoclastic to H2-Dependent Methanogenesis in a West Siberian Peat Bog at Low pH Values and Isolation of an Acidophilic Methanobacterium Strain , 2007, Applied and Environmental Microbiology.

[4]  D. Stahl,et al.  Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens , 1994, Applied and environmental microbiology.

[5]  E. E. L O G A N,et al.  Direct Biological Conversion of Electrical Current into Methane by Electromethanogenesis , 2009 .

[6]  Daisuke Mayumi,et al.  Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan). , 2011, Environmental microbiology.

[7]  E. E. L O G A N,et al.  Hydrogen Production in a Single Chamber Microbial Electrolysis Cell Lacking a Membrane , 2008 .

[8]  Willy Verstraete,et al.  Methanogenesis in membraneless microbial electrolysis cells , 2009, Applied Microbiology and Biotechnology.

[9]  F. Zhao,et al.  Effect of pH on sulfate removal from wastewater using a bioelectrochemical system , 2013 .

[10]  Mauro Majone,et al.  Bioelectrochemical reduction of CO(2) to CH(4) via direct and indirect extracellular electron transfer by a hydrogenophilic methanogenic culture. , 2010, Bioresource technology.

[11]  H. Hamelers,et al.  Principle and perspectives of hydrogen production through biocatalyzed electrolysis , 2006 .

[12]  Bruce E Logan,et al.  Sustainable and efficient biohydrogen production via electrohydrogenesis , 2007, Proceedings of the National Academy of Sciences.

[13]  E. E. L O G A N,et al.  Electrochemically Assisted Microbial Production of Hydrogen from Acetate , 2022 .

[14]  B. Demirel,et al.  The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review , 2008 .

[15]  S H A O A N C H E N G, † H U B E R T U,et al.  Microbial Electrolysis Cells for High Yield Hydrogen Gas Production from Organic Matter , 2008 .

[16]  M. H. Chakrabarti,et al.  A review on the effect of bio-electrodes on denitrification and organic matter removal processes in bio-electrochemical systems , 2013 .

[17]  W. Liu,et al.  Characterization of microbial consortia in a terephthalate-degrading anaerobic granular sludge system. , 2001, Microbiology.

[18]  N. Ren,et al.  Enhanced hydrogen production from waste activated sludge by cascade utilization of organic matter in microbial electrolysis cells. , 2012, Water research.

[19]  Reyes Sierra-Alvarez,et al.  Zero valent iron as an electron-donor for methanogenesis and sulfate reduction in anaerobic sludge. , 2005, Biotechnology and bioengineering.

[20]  W Verstraete,et al.  Combining biocatalyzed electrolysis with anaerobic digestion. , 2008, Water science and technology : a journal of the International Association on Water Pollution Research.

[21]  Aijie Wang,et al.  A rapid selection strategy for an anodophilic consortium for microbial fuel cells. , 2010, Bioresource technology.

[22]  Robert C. Wolpert,et al.  A Review of the , 1985 .

[23]  Daisuke Sasaki,et al.  Bioelectrochemical system stabilizes methane fermentation from garbage slurry. , 2010, Bioresource technology.

[24]  Pinjing He,et al.  Predominant contribution of syntrophic acetate oxidation to thermophilic methane formation at high acetate concentrations. , 2011, Environmental science & technology.

[25]  Huimin Zhao,et al.  Applying an electric field in a built-in zero valent iron--anaerobic reactor for enhancement of sludge granulation. , 2011, Water research.

[26]  P. He,et al.  Self-adaption of methane-producing communities to pH disturbance at different acetate concentrations by shifting pathways and population interaction. , 2013, Bioresource technology.

[27]  A. Stams,et al.  Electricity-mediated biological hydrogen production. , 2010, Current opinion in microbiology.

[28]  K. S. Creamer,et al.  Inhibition of anaerobic digestion process: a review. , 2008, Bioresource technology.

[29]  Hubertus V M Hamelers,et al.  Effect of operational parameters on Coulombic efficiency in bioelectrochemical systems. , 2011, Bioresource technology.

[30]  Irini Angelidaki,et al.  Effect of ammonium and acetate on methanogenic pathway and methanogenic community composition. , 2013, FEMS microbiology ecology.

[31]  Hideki Harada,et al.  Fluorescence In Situ Hybridization Using 16S rRNA-Targeted Oligonucleotides Reveals Localization of Methanogens and Selected Uncultured Bacteria in Mesophilic and Thermophilic Sludge Granules , 1999, Applied and Environmental Microbiology.

[32]  Kyu-Jung Chae,et al.  Selective inhibition of methanogens for the improvement of biohydrogen production in microbial electrolysis cells , 2010 .

[33]  Irini Angelidaki,et al.  Acetate Oxidation Is the Dominant Methanogenic Pathway from Acetate in the Absence of Methanosaetaceae , 2006, Applied and Environmental Microbiology.

[34]  Qi Zhou,et al.  Effect of sodium dodecyl sulfate on waste activated sludge hydrolysis and acidification , 2007 .

[35]  S. Hattori.  Syntrophic acetate-oxidizing microbes in methanogenic environments. , 2008, Microbes and environments.

[36]  Fan Lü,et al.  Shift of pathways during initiation of thermophilic methanogenesis at different initial pH. , 2012, Bioresource technology.

[37]  Ralf Cord-Ruwisch,et al.  Novel methanogenic rotatable bioelectrochemical system operated with polarity inversion. , 2011, Environmental science & technology.