Complete structure of the chloroplast genome of Arabidopsis thaliana.

The complete nucleotide sequence of the chloroplast genome of Arabidopsis thaliana has been determined. The genome as a circular DNA composed of 154,478 bp containing a pair of inverted repeats of 26,264 bp, which are separated by small and large single copy regions of 17,780 bp and 84,170 bp, respectively. A total of 87 potential protein-coding genes including 8 genes duplicated in the inverted repeat regions, 4 ribosomal RNA genes and 37 tRNA genes (30 gene species) representing 20 amino acid species were assigned to the genome on the basis of similarity to the chloroplast genes previously reported for other species. The translated amino acid sequences from respective potential protein-coding genes showed 63.9% to 100% sequence similarity to those of the corresponding genes in the chloroplast genome of Nicotiana tabacum, indicating the occurrence of significant diversity in the chloroplast genes between two dicot plants. The sequence data and gene information are available on the World Wide Web database KAOS (Kazusa Arabidopsis data Opening Site) at http://www.kazusa.or.jp/arabi/.

[1]  Susan E. Douglas,et al.  The Plastid Genome of the Cryptophyte Alga, Guillardia theta: Complete Sequence and Conserved Synteny Groups Confirm Its Common Ancestry with Red Algae , 1999, Journal of Molecular Evolution.

[2]  J. Cherry,et al.  Arabidopsis thaliana: a model plant for genome analysis. , 1998, Science.

[3]  M. Hasegawa,et al.  Gene transfer to the nucleus and the evolution of chloroplasts , 1998, Nature.

[4]  T. Kuroiwa,et al.  ANALYSES OF THE PLASTID GENOME OF PROTOFLORIDEOPHYCEOUS ALGAE , 1998 .

[5]  T. Wakasugi.  Complete nucleotide sequence of the plastid genome from a fern, Psilotum nudum , 1998 .

[6]  M. Sugiura,et al.  Evolution and mechanism of translation in chloroplasts. , 1998, Annual review of genetics.

[7]  Y. Suzuki,et al.  Complete nucleotide sequence of the chloroplast genome from the green alga Chlorella vulgaris: the existence of genes possibly involved in chloroplast division. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[8]  S. Eddy,et al.  tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. , 1997, Nucleic acids research.

[9]  Y. Nakamura,et al.  Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones. , 1997, DNA research : an international journal for rapid publication of reports on genes and genomes.

[10]  J. Kaiser.  First Global Sequencing Effort Begins , 1996, Science.

[11]  Sayaka,et al.  Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. , 1996, DNA research : an international journal for rapid publication of reports on genes and genomes.

[12]  Y. Nakamura,et al.  Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions (supplement). , 1996, DNA research : an international journal for rapid publication of reports on genes and genomes.

[13]  G. Igloi,et al.  Complete sequence of the maize chloroplast genome: gene content, hotspots of divergence and fine tuning of genetic information by transcript editing. , 1995, Journal of molecular biology.

[14]  N. Mitsukawa,et al.  Generation of a high-quality P1 library of Arabidopsis suitable for chromosome walking , 1995 .

[15]  M. Sugiura,et al.  Loss of all ndh genes as determined by sequencing the entire chloroplast genome of the black pine Pinus thunbergii. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. Monfort,et al.  Complete sequence of Euglena gracilis chloroplast DNA. , 1993, Nucleic acids research.

[17]  J. Palmer,et al.  Function and evolution of a minimal plastid genome from a nonphotosynthetic parasitic plant. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M. Sugiura,et al.  The chloroplast genome. , 1992, Plant molecular biology.

[19]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[20]  A. Prombona,et al.  A new rearrangement of angiosperm chloroplast DNA in rye (Secale cereale) involving translocation and duplication of the ribosomal rpS15 gene. , 1989, The Journal of biological chemistry.

[21]  R. Wu,et al.  Organization and nucleotide sequence of genes at both junctions between the two inverted repeats and the large single-copy region in the rice chloroplast genome. , 1988, Gene.

[22]  T. Kohchi,et al.  Genetic system of chloroplasts. , 1987, Cold Spring Harbor symposia on quantitative biology.

[23]  F. Takaiwa,et al.  The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression , 1986, The EMBO journal.

[24]  T. Kohchi,et al.  Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA , 1986, Nature.

[25]  P. Kroth-Pancic.  The Chloroplast Genome of a Chlorophyll a + c-Containing Alga , 2022 .