An open-source and low-cost feeding system for zebrafish facilities

Zebrafish is an established animal model used in the fields of developmental biology and genetics for more than 60 years, and among the first models to become genetically tractable. More recently, zebrafish has also become a model of reference for human diseases and systems neuroscience. The current extensive use of zebrafish in research and pharmaceutical companies promoted the development of several commercial husbandry systems specially designed for zebrafish. However, feeding is still a challenging and arduous task that can result in occupational disorders of the personnel working at the zebrafish facilities (e.g. tendinitis, back pain, etc.). To palliate these risks, a commercial robotic approach has been developed, yet its expensive cost makes this solution accessible only to very large fish facilities. Most mid-size and small facilities with limited resources still use manual feeding methods. Here, we propose two custom-made open-source semi-automatic feeding systems for dry and live food, capable of preventing and/or alleviating occupational disorders, improve feeding accuracy and decrease feeding time. Both systems are designed for mid-size or small fish facilities. They are cheap and can be easily and rapidly built using 3D printing and standard electronic and lab components.