论文信息 - 5D-intravital tomography as a novel tool for non-invasive in-vivo analysis of human skin

5D-intravital tomography as a novel tool for non-invasive in-vivo analysis of human skin

Some years ago, CE-marked clinical multiphoton systems for 3D imaging of human skin with subcellular resolution have been launched. These tomographs provide optical biopsies with submicron resolution based on two-photon excited autofluorescence (NAD(P)H, flavoproteins, keratin, elastin, melanin, porphyrins) and second harmonic generation by collagen. The 3D tomograph was now transferred into a 5D imaging system by the additional detection of the emission spectrum and the fluorescence lifetime based on spatially and spectrally resolved time-resolved single photon counting. The novel 5D intravital tomograph (5D-IVT) was employed for the early detection of atopic dermatitis and the analysis of treatment effects.

[1] Karsten König,et al. Intravital multiphoton tomography as a novel tool for non-invasive in vivo analysis of human skin affected with atopic dermatitis , 2010, BiOS.

[2] K. König,et al. Two-photon microscopes and in vivo multiphoton tomographs--powerful diagnostic tools for tissue engineering and drug delivery. , 2006, Advanced drug delivery reviews.

[3] Iris Riemann,et al. High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. , 2003, Journal of biomedical optics.

[4] Karsten König,et al. In vivo assessment of human skin aging by multiphoton laser scanning tomography. , 2006, Optics letters.

[5] Karsten König,et al. Clinical multiphoton tomography and clinical two-photon microendoscopy , 2009, BiOS.

[6] Karsten König,et al. Sensitivity and specificity of multiphoton laser tomography for in vivo and ex vivo diagnosis of malignant melanoma. , 2009, The Journal of investigative dermatology.

[7] I. Riemann,et al. In vivo Drug Screening in Human Skin Using Femtosecond Laser Multiphoton Tomography , 2006, Skin Pharmacology and Physiology.

[8] Watt W Webb,et al. Interpreting second-harmonic generation images of collagen I fibrils. , 2005, Biophysical journal.

[9] Karsten König,et al. Multiphoton microscopy for the investigation of dermal penetration of nanoparticle-borne drugs. , 2006, The Journal of investigative dermatology.