Methods for Analyzing miRNA Expression

Abstract This chapter describes preanalytic variables that may strongly impact on the final result of miRNA analysis and methods for selective purification and analysis of small RNAs. Emphasis is given on measuring miRNAs in matrices, tissue samples, and cell culture, as the starting material might determine the choice of the most appropriate technical strategy. Further, this chapter gives special considerations for profiling miRNA expression on the global and target-specific scales. Taking into account the different aspects characterizing the challenges of miRNA analysis, the chapter will discuss the pros and cons of the techniques currently available and will try to provide the basic knowledge necessary to choose the most appropriate analysis workflow.