In this paper, we proposed the single-channel adaptive noise canceller for the enhancement of heart sound (HS) in the auscultation signal. In case of either normal or emergency, a HS diagnosis is difficult due to the various signal source in the chest. Therefore, the HS enhancement is necessary. The conventional active noise canceller(ANC) has two channel, main signal and reference signal. For signal channel, the reference signal in ANC was generated by the proposed HS analyser and BS-Gate based on the characteristic of HS. This reference signal is suitable to the ANC condition. Experimental data were acquisited from MP36, SS30L in BIOPAC Inc., By the experiment, we confirmed that the proposed single-channel ANC was efficient for HS enhancement. And by the comparison with active linear enhancement, it was validate that the proposed ANC is not affected by the variation of a heartbeat.