Pinpoint injection of microtools for minimally invasive micromanipulation of microbe by laser trap

This paper reports transportation of the target microbe by the laser trapped microtools with minimum laser irradiation to the target. The size of a microtool (MT) is around micrometer. The MTs are manipulated by the focused laser under the microscope to manipulate the target microbe. Here we propose a pinpoint injection method of MTs at the desired location in the microchamber, which is filled with liquid. At first, we classified the injection method of the MTs in four categories. Here we employed a new method to install the MTs inside the microchamber. We developed a MT holding chip to install the MTs. The MTs were injected in the microchamber, and were manipulated successfully by the laser scanning micromanipulator to transport the target microbe for separation. The proposed method is useful for the pinpoint injection of MTs and separation by the indirect micromanipulation.

[1]  Ryutaro Maeda,et al.  A pneumatically-actuated three-way microvalve fabricated with polydimethylsiloxane using the membrane transfer technique , 2000 .

[2]  Tomokazu Matsue,et al.  Rapid micropatterning of living cells by repulsive dielectrophoretic force , 1997 .

[3]  Fumihito Arai,et al.  High speed random separation of microobject in microchip by laser manipulator and dielectrophoresis , 2000, Proceedings IEEE Thirteenth Annual International Conference on Micro Electro Mechanical Systems (Cat. No.00CH36308).

[4]  T. Katsuragi,et al.  Screening for microorganisms with specific characteristics by flow cytometry and single-cell sorting. , 2000, Journal of bioscience and bioengineering.

[5]  F. Arai,et al.  Pinpoint injection of micro tools using dielectrophoresis and hydrophobic surface for minimally invasive separation of microbe , 2002, Technical Digest. MEMS 2002 IEEE International Conference. Fifteenth IEEE International Conference on Micro Electro Mechanical Systems (Cat. No.02CH37266).

[6]  Fumihito Arai,et al.  High‐speed separation system of randomly suspended single living cells by laser trap and dielectrophoresis , 2001, Electrophoresis.

[7]  H. Misawa,et al.  Pattern formation and flow control of fine particles by laser-scanning micromanipulation. , 1991, Optics letters.

[8]  M. Berns,et al.  Wavelength dependence of cell cloning efficiency after optical trapping. , 1996, Biophysical journal.

[9]  Younan Xia,et al.  Self‐Assembly of Monodispersed Spherical Colloids into Complex Aggregates with Well‐Defined Sizes, Shapes, and Structures , 2001 .

[10]  Fumihito Arai,et al.  Stagnation point control by pressure balancing in microchannel for high speed and high purity separation of microobject , 2001, Proceedings 2001 IEEE/RSJ International Conference on Intelligent Robots and Systems. Expanding the Societal Role of Robotics in the the Next Millennium (Cat. No.01CH37180).

[11]  Fumihito Arai,et al.  Indirect manipulation and bilateral control of the microbe by the laser manipulated microtools , 2000, Proceedings. 2000 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2000) (Cat. No.00CH37113).

[12]  J. Voldman,et al.  Holding forces of single-particle dielectrophoretic traps. , 2001, Biophysical journal.

[13]  Fumihito Arai,et al.  Minimally invasive micromanipulation of microbe by laser trapped micro tools , 2002, Proceedings 2002 IEEE International Conference on Robotics and Automation (Cat. No.02CH37292).

[14]  Hywel Morgan,et al.  Dielectrophoretic separation of nano-particles , 1997 .

[15]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[16]  Fumihito Arai,et al.  Screening of single Escherichia coli in a microchannel system by electric field and laser tweezers , 1998 .

[17]  J. Aizenberg,et al.  Patterned colloidal deposition controlled by electrostatic and capillary forces. , 2000, Physical review letters.

[18]  Yoshitake Masuda,et al.  Arrangement of Nanosized Ceramic Particles on Self-Assembled Monolayers , 2000 .