Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants

Abstract In order to find out new sources of safe and inexpensive antioxidants, the antioxidant capacities of 45 selected medicinal plants were evaluated using ferric reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) assays, respectively, and the total phenolic contents of these plants were measured by the Folin–Ciocalteu method. Most of these plants were analyzed for the first time for their antioxidant activities. It was found that the plants Sargentodoxa cuneata Rehd. Et Wils, Fraxinus rhynchophylla Hance, Paeonia lactiflora Pall, Paeonia suffruticosa Andr and Scutellaria baicalensis Ceorgi possessed the highest antioxidant capacities and thus could be potential rich sources of natural antioxidants. A strong correlation between TEAC values and those obtained from FRAP assay implied that antioxidants in these plants were capable of scavenging free radicals and reducing oxidants. A high correlation between antioxidant capacities and their total phenolic contents indicated that phenolic compounds were a major contributor of antioxidant activity of these plants.

[1]  N. Holbrook,et al.  Oxidants, oxidative stress and the biology of ageing , 2000, Nature.

[2]  Luis Cisneros-Zevallos,et al.  Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products. , 2003, Journal of agricultural and food chemistry.

[3]  R. Liu,et al.  Nutrition: Antioxidant activity of fresh apples , 2000, Nature.

[4]  Hyun Pyo Kim,et al.  Antioxidative constituents fromPaeonia lactiflora , 2005 .

[5]  Feng Chen,et al.  Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography. , 2005, Journal of chromatography. A.

[6]  C. Wei,et al.  Tannins and human health: a review. , 1998, Critical reviews in food science and nutrition.

[7]  L. Leong,et al.  Antioxidant activities of aqueous extracts of selected plants , 2006 .

[8]  Feng Chen,et al.  A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay , 2006 .

[9]  Kaixun Huang,et al.  Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. , 1999, Biochimica et biophysica acta.

[10]  Feng Chen,et al.  Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae , 2007 .

[11]  Mei Sun,et al.  Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer , 2004, Life Sciences.

[12]  Feng Chen,et al.  Separation methods used for Scutellaria baicalensis active components , 2004, Journal of Chromatography B.

[13]  J J Strain,et al.  The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. , 1996, Analytical biochemistry.

[14]  Dejian Huang,et al.  When east meets west: the relationship between yin‐yang and antioxidation‐oxidation , 2003, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[15]  C. Teng,et al.  Phytochemical and Pharmacological Studies on Chinese Paeonia Species , 2000 .

[16]  F. Liu,et al.  Antioxidative and free radical scavenging activities of selected medicinal herbs. , 2000, Life sciences.

[17]  D. K. Salunkhe,et al.  Food Antioxidants: Technological: Toxicological and Health Perspectives , 1995 .

[18]  Andrew Jenner,et al.  Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional chinese medicines. , 2004, Free radical biology & medicine.

[19]  C. Rice-Evans,et al.  Antioxidant activity applying an improved ABTS radical cation decolorization assay. , 1999, Free radical biology & medicine.

[20]  Feng Chen,et al.  Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography. , 2004, Journal of chromatography. A.

[21]  K. Gey The antioxidant hypothesis of cardiovascular disease: epidemiology and mechanisms. , 1990, Biochemical Society transactions.

[22]  P. Kuo,et al.  Chemical constituents of the stem of Sargentodoxa cuneata , 2003 .

[23]  R. Blomhoff,et al.  Several culinary and medicinal herbs are important sources of dietary antioxidants. , 2003, The Journal of nutrition.

[24]  N Ito,et al.  Studies on antioxidants: their carcinogenic and modifying effects on chemical carcinogenesis. , 1986, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[25]  H. Hwang,et al.  Screening of medicinal plant extracts for antioxidant activity. , 2003, Life sciences.

[26]  C. C. Lin,et al.  Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. , 2000, Anticancer research.

[27]  J. Ríos,et al.  Antioxidant activity of anti-inflammatory plant extracts. , 2002, Life sciences.

[28]  A M Safer,et al.  Hepatotoxicity induced by the anti-oxidant food additive, butylated hydroxytoluene (BHT), in rats: an electron microscopical study. , 1999, Histology and histopathology.

[29]  V. L. Singleton,et al.  Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents , 1965, American Journal of Enology and Viticulture.

[30]  Hyo Jin Kim,et al.  Antioxidative Activity of Resveratrol and Its Derivatives Isolated from Seeds of Paeonia lactiflora , 2002, Bioscience, biotechnology, and biochemistry.

[31]  C. la Vecchia,et al.  Vegetables, fruit, antioxidants and cancer: a review of Italian studies , 2001, European journal of nutrition.