论文信息 - Quasi white light multiphoton imaging

Quasi white light multiphoton imaging

We describe the realization and characterization of a broadband, high power density and fully spectrally controllable source, suitable for multiphoton imaging of biological samples. We used a photonic crystal fiber (PCF) with selected dispersive and non-linear properties, in order to generate, when pumped with <140 femtosecond pulses delivered by a tunable Ti:Sa laser (Chameleon Ultra II by Coherent Inc.), a smooth continuum in the 700nm-950nm region, with average power density grater than 2mW/nm. Time distribution of the generated spectrum has been measured with autocorrelation technique. Axial and lateral resolution obtained with a scanning multiphoton system has been determined to be near the theoretical limit. The possibility of two-photon excitation of different dyes in the same sample and high image resolution are demonstrated at tens of microns in depth. Future developments and different applications are also discussed.

[1] M. Neil,et al. Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging. , 2008, Optics letters.

[2] J. Dudley,et al. Supercontinuum generation in photonic crystal fiber , 2006 .

[3] Colin J. R. Sheppard,et al. Image formation in two-photon fluorescence microscopy , 1990 .

[4] W. Webb,et al. Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence. , 1997, Applied optics.

[5] T. A. Birks,et al. Widely tunable femtosecond pulses from a tapered fiber for ultrafast microscopy and multiphoton applications , 2002 .

[6] W. Webb,et al. Nonlinear magic: multiphoton microscopy in the biosciences , 2003, Nature Biotechnology.

[7] S. Leon-Saval,et al. Supercontinuum generation system for optical coherence tomography based on tapered photonic crystal fibre. , 2006, Optics express.

[8] O. Nakamura,et al. Fundamental of two‐photon microscopy , 1999, Microscopy research and technique.

[9] C. Dunsby,et al. An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy , 2004, (CLEO). Conference on Lasers and Electro-Optics, 2005..