Efficiency Determination and Mechanism Investigation of Autotrophic Denitrification Strain F1 to Promote Low-Carbon Development

: Shewanella sp. strain F1, isolated from a lab-scale Fe(II) − dependent anaerobic denitrifying reactor, could reduce nitrate by oxidizing Fe(II). Its nitrate reduction rate and Fe(II) oxidation rate were 0.48 mg/(L · h) and 5.05 mg/(L · h) at OD 600 of 0.4786 with a five-fold diluent. Shewanella sp. was popular in Fe(III) reduction. Fewer studies about its ability for Fe(II) oxidation are available. The low pH was determined to be the switch for Shewanella sp. strain F1 to perform Fe(III) reduction or Fe(II) oxidation. Even under a low pH, the produced Fe(III) precipitated around cells from iron encrustation. By observation of the morphologies of strain F1, two corresponding microbial mechanisms were proposed. One was named Cyc 2 − based Fe(II)-dependent denitrification, in which Fe(II) was oxidized by Cyc 2 in the outer cell membrane, and the produced Fe(III) precipitated on the cell wall surface to form tiled iron encrustation. The other was named Cyc 1 − based Fe(II) − dependent denitrification, in which Fe(II) was oxidized on the existing iron precipitation on the cell wall surface to form towery iron encrustation, and the electron was transported to Cyc 1 in the periplasm. The efficiency determination and mechanism investigation of strain F1 will promote the development of autotrophic denitrification technology and meet the requirement of a low − carbon policy.

[1]  Tinglin Huang,et al.  Bacterial community structure and metabolic activity of drinking water pipelines in buildings: A new perspective on dual effects of hydrodynamic stagnation and algal organic matter invasion. , 2022, Water research.

[2]  L. Young,et al.  Metagenomic analysis of Fe(II)-oxidizing bacteria for Fe(III) mineral formation and carbon assimilation under microoxic conditions in paddy soil. , 2022, The Science of the total environment.

[3]  R. Amils,et al.  Shewanella sp. T2.3D-1.1 a Novel Microorganism Sustaining the Iron Cycle in the Deep Subsurface of the Iberian Pyrite Belt , 2022, Microorganisms.

[4]  Yinguang Chen,et al.  Enhancement of hexavalent chromium reduction by Shewanella oneidensis MR-1 in presence of copper nanoparticles via stimulating bacterial extracellular electron transfer and environmental adaptability. , 2022, Bioresource technology.

[5]  Chen Wang,et al.  Culture-dependent and culture-independent methods reveal microbe-clay mineral interactions by dissimilatory iron-reducing bacteria in an integral oilfield. , 2022, The Science of the total environment.

[6]  Yingqi Lu,et al.  Effect of asparagine, corncob biochar and Fe(II) on anaerobic biological treatment under low temperature: Enhanced performance and microbial community dynamic. , 2022, Journal of environmental management.

[7]  Jihoon Shin,et al.  Simultaneous feature engineering and interpretation: Forecasting harmful algal blooms using a deep learning approach. , 2022, Water research.

[8]  Wenxuan Li,et al.  Use of Sponge Iron as an Indirect Electron Donor to Provide Ferrous Iron for Nitrate-Dependent Ferrous Oxidation Processes: Denitrification Performance and Mechanism , 2022, SSRN Electronic Journal.

[9]  Xiang Li,et al.  Function of Fe(III)-minerals in the enhancement of anammox performance exploiting integrated network and metagenomics analyses. , 2021, Water research.

[10]  Yong-feng Jia,et al.  Effect of co-existent Al(III) in As-rich Acid Mine Drainage (AMD) on As removal during Fe(II) and As(III) abiotic oxidation process , 2021, Journal of Water Process Engineering.

[11]  Long Zou,et al.  Biogenic iron sulfide functioning as electron-mediating interface to accelerate dissimilatory ferrihydrite reduction by Shewanella oneidensis MR-1. , 2021, Chemosphere.

[12]  Bin Ma,et al.  Achieving advanced nitrogen removal in a novel partial denitrification/anammox-nitrifying (PDA-N) biofilter process treating low C/N ratio municipal wastewater. , 2021, Bioresource technology.

[13]  G. Qian,et al.  Nitrate removal during Fe(III) bio-reduction in microbial-mediated iron redox cycling systems , 2021, Water Science and Technology.

[14]  Q. Mahmood,et al.  Recovering Phosphate and Energy from Anaerobic Sludge Digested Wastewater with Iron-Air Fuel Cells: Two-Chamber Cell Versus One-Chamber Cell , 2021, SSRN Electronic Journal.

[15]  Jianghua Yu,et al.  Microbial interspecific interaction and nitrogen metabolism pathway for the treatment of municipal wastewater by iron carbon based constructed wetland. , 2020, Bioresource technology.

[16]  P. Zheng,et al.  Iron as electron donor for denitrification: The efficiency, toxicity and mechanism. , 2020, Ecotoxicology and environmental safety.

[17]  R. Jia,et al.  Abundance and community succession of nitrogen-fixing bacteria in ferrihydrite enriched cultures of paddy soils is closely related to Fe(III)-reduction. , 2020, The Science of the total environment.

[18]  Yongzhen Peng,et al.  Enhanced long-term advanced denitrogenation from nitrate wastewater by anammox consortia: Dissimilatory nitrate reduction to ammonium (DNRA) coupling with anammox in an upflow biofilter reactor equipped with EDTA-2Na/Fe(II) ratio and pH control. , 2020, Bioresource technology.

[19]  He-ping Zhao,et al.  Structures of nitroaromatic compounds induce Shewanella oneidensis MR-1 to adopt different electron transport pathways to reduce the contaminants. , 2019, Journal of hazardous materials.

[20]  Tinglin Huang,et al.  Microbial Community Analysis and Effect of nZVI on Autotrophic Denitrification in a Biological Reactor , 2019, Environmental Engineering Science.

[21]  Y. Liu,et al.  Effect of Fe(II) on reactivity of heterotrophic denitrifiers in the remediation of nitrate- and Fe(II)-contaminated groundwater. , 2018, Ecotoxicology and environmental safety.

[22]  A. Singh,et al.  Iron oxidizing bacteria: insights on diversity, mechanism of iron oxidation and role in management of metal pollution , 2018, Environmental Sustainability.

[23]  Lei Wang,et al.  Engineered Shewanella oneidensis-reduced graphene oxide biohybrid with enhanced biosynthesis and transport of flavins enabled a highest bioelectricity output in microbial fuel cells , 2018, Nano Energy.

[24]  P. Zheng,et al.  Chemolithotrophic denitrification by nitrate-dependent anaerobic iron oxidizing (NAIO) process: Insights into the evaluation of seeding sludge , 2018, Chemical Engineering Journal.

[25]  R. Louro,et al.  Extracellular reduction of solid electron acceptors by Shewanella oneidensis , 2018, Molecular microbiology.

[26]  Fangbai Li,et al.  Biological and chemical processes of microbially mediated nitrate-reducing Fe(II) oxidation by Pseudogulbenkiania sp. strain 2002 , 2018 .

[27]  H. Bastani,et al.  Change regularity of water quality parameters in leakage flow conditions and their relationship with iron release. , 2017, Water research.

[28]  P. Zheng,et al.  Chemoautotrophic denitrification based on ferrous iron oxidation: Reactor performance and sludge characteristics , 2017 .

[29]  Yong-guan Zhu,et al.  Electron shuttle-mediated microbial Fe(III) reduction under alkaline conditions , 2017, Journal of Soils and Sediments.

[30]  P. Zheng,et al.  Physicochemical characteristics and microbial community of cultivated sludge for nitrate-dependent anaerobic ferrous-oxidizing (NAFO) process , 2016 .

[31]  Hanqing Yu,et al.  Interaction between ferrihydrite and nitrate respirations by Shewanella oneidensis MR-1 , 2015 .

[32]  Jun Zhou,et al.  Aerobic denitrification: A review of important advances of the last 30 years , 2015, Biotechnology and Bioprocess Engineering.

[33]  Tinglin Huang,et al.  Anaerobic nitrate-dependent iron(II) oxidation by a novel autotrophic bacterium, Pseudomonas sp. SZF15 , 2015 .

[34]  Jun Zhou,et al.  Nitrate removal by nitrate-dependent Fe(II) oxidation in an upflow denitrifying biofilm reactor. , 2015, Water science and technology : a journal of the International Association on Water Pollution Research.

[35]  P. Zheng,et al.  Performance of nitrate-dependent anaerobic ferrous oxidizing (NAFO) process: a novel prospective technology for autotrophic denitrification. , 2015, Bioresource technology.

[36]  Hye Suk Byun,et al.  Shewanella oneidensis MR-1 nanowires are outer membrane and periplasmic extensions of the extracellular electron transport components , 2014, Proceedings of the National Academy of Sciences.

[37]  Y. Stierhof,et al.  3‐D analysis of bacterial cell‐(iron)mineral aggregates formed during Fe(II) oxidation by the nitrate‐reducing Acidovorax sp. strain BoFeN1 using complementary microscopy tomography approaches , 2014, Geobiology.

[38]  P. Zheng,et al.  Partitionable-space enhanced coagulation (PEC) reactor and its working mechanism: a new prospective chemical technology for phosphorus pollution control. , 2014, Water research.

[39]  K. Rosso,et al.  Mtr extracellular electron-transfer pathways in Fe(III)-reducing or Fe(II)-oxidizing bacteria: a genomic perspective. , 2012, Biochemical Society transactions.