Positional assembly of enzymes in polymersome nanoreactors for cascade reactions.

In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block-copolymer membrane of polymersomes. A one-pot multistep reaction sequence is performed with the nanoreactor in combination with free Candida antarctica lipase B (CALB) in the bulk solution (see picture; ABTS: 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)). (Chemical Equation Presented)

[1]  Kenichi Yoshikawa,et al.  Gene Expression within Cell‐Sized Lipid Vesicles , 2003, Chembiochem : a European journal of chemical biology.

[2]  Kurt Faber,et al.  Biotransformations in Organic Chemistry , 1992 .

[3]  R. Nolte,et al.  Electroformed giant vesicles from thiophene-containing rod-coil diblock copolymers , 2004 .

[4]  D. Avnir,et al.  Recent bio-applications of sol–gel materials , 2006 .

[5]  Huangxian Ju,et al.  Immobilization of Biomolecules in Sol–Gels: Biological and Analytical Applications , 2006 .

[6]  P. Luisi,et al.  Protein expression in liposomes. , 1999, Biochemical and biophysical research communications.

[7]  J. Wu,et al.  Proton diffusion across membranes of vesicles of poly(styrene-b-acrylic acid) diblock copolymers. , 2006, Journal of the American Chemical Society.

[8]  D. Hammer,et al.  Quantitative membrane loading of polymer vesicles. , 2006, Soft matter.

[9]  S. Ichikawa,et al.  Enzymes inside lipid vesicles: preparation, reactivity and applications. , 2001, Biomolecular engineering.

[10]  D. Hammer,et al.  Cross-linked polymersome membranes: Vesicles with broadly adjustable properties , 2002 .

[11]  Dennis E. Discher,et al.  Polymer vesicles : Materials science: Soft surfaces , 2002 .

[12]  Hyo-Jick Choi,et al.  Artificial organelle: ATP synthesis from cellular mimetic polymersomes. , 2005, Nano letters.

[13]  Johannes A A W Elemans,et al.  Self-assembled nanoreactors. , 2005, Chemical reviews.

[14]  A. Eisenberg,et al.  Thermodynamic Size Control of Block Copolymer Vesicles in Solution , 2001 .

[15]  T. Oberholzer,et al.  Giant Vesicles as Microreactors for Enzymatic mRNA Synthesis , 2002, Chembiochem : a European journal of chemical biology.

[16]  Mathias Winterhalter,et al.  A nanocompartment system (Synthosome) designed for biotechnological applications. , 2006, Journal of biotechnology.

[17]  D. Hammer,et al.  Bioresorbable Vesicles Formed through Spontaneous Self-Assembly of Amphiphilic Poly(ethylene oxide)-block-polycaprolactone. , 2006, Macromolecules.

[18]  Frank Caruso,et al.  Layer-by-layer engineered capsules and their applications , 2006 .

[19]  R. Nolte,et al.  Vesicles and polymerized vesicles from thiophene-containing rod-coil block copolymers. , 2003, Angewandte Chemie.