Comparison of Oxidative Properties, Light Absorbance, and Total and Elemental Mass Concentration of Ambient PM2.5 Collected at 20 European Sites

Objective It has been proposed that the redox activity of particles may represent a major determinant of their toxicity. We measured the in vitro ability of ambient fine particles [particulate matter with aerodynamic diameters ≤2.5 μm (PM2.5)] to form hydroxyl radicals (•OH) in an oxidant environment, as well as to deplete physiologic antioxidants (ascorbic acid, glutathione) in the naturally reducing environment of the respiratory tract lining fluid (RTLF). The objective was to examine how these toxicologically relevant measures were related to other PM characteristics, such as total and elemental mass concentration and light absorbance. Design Gravimetric PM2.5 samples (n = 716) collected over 1 year from 20 centers participating in the European Community Respiratory Health Survey were available. Light absorbance of these filters was measured with reflectometry. PM suspensions were recovered from filters by vortexing and sonication before dilution to a standard concentration. The oxidative activity of these particle suspensions was then assessed by measuring their ability to generate •OH in the presence of hydrogen peroxide, using electron spin resonance and 5,5-dimethyl-1-pyrroline-N-oxide as spin trap, or by establishing their capacity to deplete antioxidants from a synthetic model of the RTLF. Results and Conclusion PM oxidative activity varied significantly among European sampling sites. Correlations between oxidative activity and all other characteristics of PM were low, both within centers (temporal correlation) and across communities (annual mean). Thus, no single surrogate measure of PM redox activity could be identified. Because these novel measures are suggested to reflect crucial biologic mechanisms of PM, their use may be pertinent in epidemiologic studies. Therefore, it is important to define the appropriate methods to determine oxidative activity of PM.

[1]  T. Moreno,et al.  Pulmonary antioxidants exert differential protective effects against urban and industrial particulate matter , 2003, Journal of Biosciences.

[2]  J Pekkanen,et al.  Associations between ambient, personal, and indoor exposure to fine particulate matter constituents in Dutch and Finnish panels of cardiovascular patients , 2005, Occupational and Environmental Medicine.

[3]  H. Zechmeister,et al.  Estimation of element deposition derived from road traffic sources by using mosses. , 2005, Environmental pollution.

[4]  Susan J Wilson,et al.  Diesel exhaust activates redox-sensitive transcription factors and kinases in human airways. , 2005, American journal of physiology. Lung cellular and molecular physiology.

[5]  W. MacNee,et al.  Combustion-derived nanoparticles: A review of their toxicology following inhalation exposure , 2005, Particle and Fibre Toxicology.

[6]  D. Jarvis,et al.  Elemental composition and reflectance of ambient fine particles at 21 European locations , 2005 .

[7]  Constantinos Sioutas,et al.  Redox activity of airborne particulate matter at different sites in the Los Angeles Basin. , 2005, Environmental research.

[8]  B. Brunekreef,et al.  Epidemiological evidence of effects of coarse airborne particles on health , 2005, European Respiratory Journal.

[9]  L. Sheppard.  Acute Air Pollution Effects: Consequences of Exposure Distribution and Measurements , 2005, Journal of toxicology and environmental health. Part A.

[10]  Thomas Lumley,et al.  Exposure and measurement contributions to estimates of acute air pollution effects , 2005, Journal of Exposure Analysis and Environmental Epidemiology.

[11]  André Nel,et al.  ATMOSPHERE: Enhanced: Air Pollution-Related Illness: Effects of Particles , 2005 .

[12]  Robin Shattock,et al.  In Vitro and In Vivo: The Story of Nonoxynol 9 , 2005, Journal of acquired immune deficiency syndromes.

[13]  J. Schwartz,et al.  Ambient Gas Concentrations and Personal Particulate Matter Exposures: Implications for Studying the Health Effects of Particles , 2005, Epidemiology.

[14]  P. Kao,et al.  Lung surfactant gelation induced by epithelial cells exposed to air pollution or oxidative stress. , 2005, American journal of respiratory cell and molecular biology.

[15]  D. Zmirou-Navier,et al.  Can we use fixed ambient air monitors to estimate population long-term exposure to air pollutants? The case of spatial variability in the Genotox ER study. , 2005, Environmental research.

[16]  Qing Yu Meng,et al.  Influence of ambient (outdoor) sources on residential indoor and personal PM2.5 concentrations: Analyses of RIOPA data , 2005, Journal of Exposure Analysis and Environmental Epidemiology.

[17]  W. Kreyling,et al.  The influence of hydrogen peroxide and histamine on lung permeability and translocation of iridium nanoparticles in the isolated perfused rat lung , 2005, Particle and Fibre Toxicology.

[18]  Paride Mantecca,et al.  Impact of tire debris on in vitro and in vivo systems , 2005, Particle and Fibre Toxicology.

[19]  Joachim Heinrich,et al.  Metal-rich ambient particles (particulate matter 2.5) cause airway inflammation in healthy subjects. , 2004, American journal of respiratory and critical care medicine.

[20]  J. Weiss,et al.  Quinones and Aromatic Chemical Compounds in Particulate Matter Induce Mitochondrial Dysfunction: Implications for Ultrafine Particle Toxicity , 2004, Environmental health perspectives.

[21]  A. Goetz,et al.  Colloidal gold particles as a new in vivo marker of early acute lung injury. , 2004, American journal of physiology. Lung cellular and molecular physiology.

[22]  Catrin Albrecht,et al.  Inhaled particles and lung cancer, part B: Paradigms and risk assessment , 2004, International journal of cancer.

[23]  Roel P F Schins,et al.  Inhaled particles and lung cancer. Part A: Mechanisms , 2004, International journal of cancer.

[24]  B. Legube,et al.  A comparative study of the effects of chloride, sulfate and nitrate ions on the rates of decomposition of H2O2 and organic compounds by Fe(II)/H2O2 and Fe(III)/H2O2. , 2004, Chemosphere.

[25]  D. Jarvis,et al.  PM2.5 and NO2 assessment in 21 European study centres of ECRHS II: annual means and seasonal differences☆ , 2004 .

[26]  F. Dominici,et al.  Time-series studies of particulate matter. , 2004, Annual review of public health.

[27]  Thomas Sandström,et al.  An in vitro and in vivo investigation of the effects of diesel exhaust on human airway lining fluid antioxidants. , 2004, Archives of biochemistry and biophysics.

[28]  R. Hillamo,et al.  Chemical and in vitro toxicologic characterization of wintertime and springtime urban-air particles with an aerodynamic diameter below 10 microm in Helsinki. , 2004, Scandinavian journal of work, environment & health.

[29]  Tingming Shi,et al.  Soluble metals as well as the insoluble particle fraction are involved in cellular DNA damage induced by particulate matter , 2004, Molecular and Cellular Biochemistry.

[30]  Thomas Kuhlbusch,et al.  Hydroxyl radical generation by electron paramagnetic resonance as a new method to monitor ambient particulate matter composition. , 2003, Journal of environmental monitoring : JEM.

[31]  Vicki Stone,et al.  Oxidative stress and calcium signaling in the adverse effects of environmental particles (PM10). , 2003, Free radical biology & medicine.

[32]  D. Jarvis,et al.  PM2.5 Assessment in 21 European Study Centers of ECRHS II: Method and First Winter Results , 2003, Journal of the Air & Waste Management Association.

[33]  P. Borm,et al.  Temporal variation of hydroxyl radical generation and 8-hydroxy-2′-deoxyguanosine formation by coarse and fine particulate matter , 2003, Occupational and environmental medicine.

[34]  A. Nel,et al.  Ultrafine particulate pollutants induce oxidative stress and mitochondrial damage. , 2002, Environmental health perspectives.

[35]  D. Jarvis,et al.  The European Community Respiratory Health Survey II , 1994, European Respiratory Journal.

[36]  Kevin R. Smith,et al.  Particle characteristics responsible for effects on human lung epithelial cells. , 2002, Research report.

[37]  D. Jarvis,et al.  The European Community Respiratory Health Survey II , 2002, European Respiratory Journal.

[38]  Yifang Zhu,et al.  Concentration and Size Distribution of Ultrafine Particles Near a Major Highway , 2002, Journal of the Air & Waste Management Association.

[39]  Matti Jantunen,et al.  Comparison of black smoke and PM2.5 levels in indoor and outdoor environments of four European cities. , 2002, Environmental science & technology.

[40]  Winston Harrington,et al.  U.S. drinking water challenges in the twenty-first century. , 2002, Environmental health perspectives.

[41]  Ning Li,et al.  USE OF A STRATIFIED OXIDATIVE STRESS MODEL TO STUDY THE BIOLOGICAL EFFECTS OF AMBIENT CONCENTRATED AND DIESEL EXHAUST PARTICULATE MATTER , 2002, Inhalation toxicology.

[42]  Antonella Zanobetti,et al.  The Temporal Pattern of Mortality Responses to Air Pollution: A Multicity Assessment of Mortality Displacement , 2002, Epidemiology.

[43]  Antonella Zanobetti,et al.  Air conditioning and source-specific particles as modifiers of the effect of PM(10) on hospital admissions for heart and lung disease. , 2001, Environmental health perspectives.

[44]  J. Froines,et al.  Versatile aerosol concentration enrichment system (VACES) for simultaneous in vivo and in vitro evaluation of toxic effects of ultrafine, fine and coarse ambient particles Part I: Development and laboratory characterization , 2001 .

[45]  S. Friedlander,et al.  Versatile aerosol concentration enrichment system (VACES) for simultaneous in vivo and in vitro evaluation of toxic effects of ultrafine, fine and coarse ambient particles Part II: Field evaluation , 2001 .

[46]  J Schwartz,et al.  Confounding and Effect Modification in the Short-Term Effects of Ambient Particles on Total Mortality: Results from 29 European Cities within the APHEA2 Project , 2001, Epidemiology.

[47]  F Neukirch,et al.  The European Community Respiratory Health Survey: what are the main results so far? European Community Respiratory Health Survey II. , 2001, The European respiratory journal.

[48]  P. Borm,et al.  Ambient particulate matter induces relaxation of rat aortic rings in vitro , 2001, Human & experimental toxicology.

[49]  J. Sarnat,et al.  Fine particulate air pollution and mortality in 20 U.S. cities. , 2001, The New England journal of medicine.

[50]  A. Nel,et al.  The role of particulate pollutants in pulmonary inflammation and asthma: evidence for the involvement of organic chemicals and oxidative stress , 2001, Current opinion in pulmonary medicine.

[51]  A. Zanobetti,et al.  Air Conditioning and Source-Specific Particles as Modifiers of the Effect of PM10 on Hospital Admissions for Heart and Lung Disease , 2001 .

[52]  Matti Jantunen,et al.  Elemental analysis of airborne particulate matter by ED-XRF within the European EXPOLIS study , 2001 .

[53]  F. Dominici,et al.  Fine particulate air pollution and mortality in 20 U.S. cities, 1987-1994. , 2000, The New England journal of medicine.

[54]  A. Ghio,et al.  Enhancement of 2'-deoxyguanosine hydroxylation and DNA damage by coal and oil fly ash in relation to particulate metal content and availability. , 2000, Chemical research in toxicology.

[55]  Martin Röösli,et al.  Validity of Ambient Levels of Fine Particles as Surrogate for Personal Exposure to Outdoor Air Pollution—Results of the European EXPOLIS-EAS Study (Swiss Center Basel) , 2000, Journal of the Air & Waste Management Association.

[56]  S L Zeger,et al.  Exposure measurement error in time-series studies of air pollution: concepts and consequences. , 2000, Environmental health perspectives.

[57]  F. Kelly,et al.  Modeling the interactions of particulates with epithelial lining fluid antioxidants. , 1999, American journal of physiology. Lung cellular and molecular physiology.

[58]  J Inmon,et al.  Ambient air particles: effects on cellular oxidant radical generation in relation to particulate elemental chemistry. , 1999, Toxicology and applied pharmacology.

[59]  V. Roginsky,et al.  Kinetics of redox interaction between substituted quinones and ascorbate under aerobic conditions. , 1999, Chemico-biological interactions.

[60]  F. Gilliland,et al.  A theoretical basis for investigating ambient air pollution and children's respiratory health. , 1999, Environmental health perspectives.

[61]  A. Ghio,et al.  Sulfate content correlates with iron concentrations in ambient air pollution particles. , 1999, Inhalation toxicology.

[62]  P. Borm,et al.  In vitro effects of coal fly ashes: hydroxyl radical generation, iron release, and DNA damage and toxicity in rat lung epithelial cells. , 1999, Inhalation toxicology.

[63]  W. MacNee,et al.  Free radical activity of PM10: iron-mediated generation of hydroxyl radicals. , 1997, Environmental health perspectives.

[64]  P. S. Gilmour,et al.  Free radical activity and pro-inflammatory effects of particulate air pollution (PM10) in vivo and in vitro. , 1996, Thorax.

[65]  K. Donaldson,et al.  Free radical activity associated with the surface of particles: a unifying factor in determining biological activity? , 1996, Toxicology letters.

[66]  G. Buettner,et al.  Catalytic metals, ascorbate and free radicals: combinations to avoid. , 1996, Radiation research.

[67]  Z. Weidenhoffer,et al.  Respiratory tract lining fluid antioxidants: the first line of defence against gaseous pollutants. , 1996 .

[68]  F. Kelly,et al.  Respiratory tract lining fluid antioxidants: the first line of defence against gaseous pollutants. , 1996, Central European journal of public health.

[69]  M. Green.  Air pollution and health , 1995 .

[70]  D. Jarvis,et al.  The European Community Respiratory Health Survey. , 1994, The European respiratory journal.

[71]  J. Zelikoff,et al.  Comparative potency of inhaled acidic sulfates: speciation and the role of hydrogen ion. , 1990, Environmental research.

[72]  B. Halliwell,et al.  Free radicals in biology and medicine , 1985 .

[73]  P. Morrow,et al.  Airway responses to sulfate and sulfuric acid aerosols in asthmatics. An exposure-response relationship. , 1983, The American review of respiratory disease.