Effect of variety and harvest date on pectin extracted from chicory roots (Cichorium intybus L.).

The influence of harvest date on pectin quality and yield of five varieties of chicory is investigated in this work. The pectin is extracted from the raw material with an acidic treatment at 85°C for an hour after inulin extraction. Main changes due to harvest date are observed in terms of neutral sugar content, average molecular weight, esterification degree and extraction yield. The galacturonic acid content remains relatively constant throughout the harvest period. While no statistically significant differences (p<0.05) in terms of galacturonic acid content and neutral sugar content are observed between the five cultivars studied, average molecular weight, esterification degree and extraction yield are significantly affected by the cultivar type. Depending on the harvest date, a broad range of pectins can easily be extracted from chicory pulps.

[1]  J. Baert.  The effect of sowing and harvest date and cultivar on inulin yield and composition of chicory (Cichorium intybus L.) roots , 1997 .

[2]  A. Voragen,et al.  Determination of the degree of methylation and acetylation of pectins by h.p.l.c. , 1986 .

[3]  G. Vanzin,et al.  Molecular genetics of nucleotide sugar interconversion pathways in plants , 2004, Plant Molecular Biology.

[4]  N. Carpita,et al.  Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. , 1993, The Plant journal : for cell and molecular biology.

[5]  Michel Paquot,et al.  Kinetic of the hydrolysis of pectin galacturonic acid chains and quantification by ionic chromatography , 2006 .

[6]  A. Voragen,et al.  Effect of enzymatic deacetylation on gelation of sugar beet pectin in the presence of calcium , 2000 .

[7]  R. Visser,et al.  Pectin — the Hairy Thing , 2003 .

[8]  S. Amaducci,et al.  Effect of harvest date and cultivar on Cichorium intybus yield components in north Italy , 1998 .

[9]  J. Harborne.  Advances in plant cell biochemistry and biotechnology , 1993 .

[10]  Bernard Courtois,et al.  Changes in esterified pectins during development in the flax stems and leaves , 2006 .

[11]  W. Van den Ende,et al.  The metabolism of fructans in roots of Cichorium intybus during growth, storage and forcing. , 1996, The New phytologist.

[12]  C. Mercier,et al.  Extraction and Charcterization of Pectins from Sugar Beet Pulp , 1985 .

[13]  M. Paquot,et al.  Use of a Plackett-Burman experimental design to examine the impact of extraction parameters on yields and compositions of pectins extracted from chicory roots (Chicorium intybus L.). , 2006, Journal of agricultural and food chemistry.

[14]  W. Willats,et al.  Side chains of pectic polysaccharides are regulated in relation to cell proliferation and cell differentiation , 1999, The Plant journal : for cell and molecular biology.

[15]  R. Visser,et al.  Advances in Pectin and Pectinase Research , 2003, Springer Netherlands.

[16]  J. Thibault,et al.  Sugar-beet pectins: Chemical structure and gelation through oxidative coupling. , 1986 .

[17]  X. Rouau,et al.  Characterisation of the extractable pectins and hemicelluloses of the cell wall of carrot , 1988 .

[18]  A. Voragen,et al.  Characterization of hop pectins shows the presence of an arabinogalactan-protein , 2002 .

[19]  R. Henry,et al.  A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS , 1983 .

[20]  R. L. Obendorf,et al.  Methanol, pectin and pectinesterase changes during soybean seed maturation , 1999, Seed Science Research.

[21]  F. Kormelink,et al.  Rhamnogalacturonase : A novel enzyme that degrades the hairy regions of pectins , 1990 .

[22]  Michel Paquot,et al.  Effect of extraction conditions on the yield, purity and surface properties of sugar beet pulp pectin extracts , 2007 .

[23]  B. Ridley,et al.  Pectins: structure, biosynthesis, and oligogalacturonide-related signaling. , 2001, Phytochemistry.

[24]  E. Bonnin,et al.  Cellulase and protease preparations can extract pectins from various plant byproducts. , 2006, Journal of agricultural and food chemistry.

[25]  P. Åman,et al.  Influence of harvest date on inulin chain length distribution and sugar profile for six chicory (Cichorium intybus L) cultivars , 1999 .

[26]  M. Paquot,et al.  New method for a two-step hydrolysis and chromatographic analysis of pectin neutral sugar chains. , 2004, Journal of agricultural and food chemistry.

[27]  Polymorphism and modulation of cell wall esterase enzyme activities in the chicory root during the growing season. , 2006, Journal of experimental botany.

[28]  J. Thibault,et al.  Characterisation of pectins extracted from fresh sugar beet under different conditions using an experimental design , 2002 .

[29]  C. Renard,et al.  STRUCTURE OF THE REPEATING UNITS IN THE RHAMNOGALACTURONIC BACKBONE OF APPLE, BEET AND CITRUS PECTINS , 1995 .

[30]  F. Sosulski,et al.  Extraction of Pectins From Sunflower Heads , 1976 .