Transcription of the segment-polarity gene wingless in the imaginal discs of Drosophila, and the phenotype of a pupal-lethal wg mutation.

Wingless (wg) is a segment-polarity gene in Drosophila which is related to the murine proto-oncogene int1. In Drosophila embryos, wg transcription defines part of each parasegment. In situ hybridization shows that wg is also expressed in the imaginal discs which give rise to the adult during metamorphosis. Transcripts are localized in the apical cytoplasm of disc cells, and accumulate in different patterns in dorsal and ventral discs. The wgCX3 mutation produces morphological defect in the adult structures derived from these imaginal discs. The results show that wg is involved in the development of the adult, as well as the embryo, but that the imaginal discs do not express this segment-polarity gene in an identical pattern to the embryonic segments.

[1]  J. Whittle.  Pattern formation in imaginal discs. , 1990, Seminars in cell biology.

[2]  S. Benzer,et al.  The sevenless + protein is expressed apically in cell membranes of developing Drosophila retina; it is not restricted to cell R7 , 1987, Cell.

[3]  Detlef Weigel,et al.  The Drosophila homology of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless , 1987, Cell.

[4]  P. Lawrence,et al.  Phenocopies induced with antisense RNA identify the wingless gene , 1987, Cell.

[5]  W. Bender,et al.  Oncogenes take wing , 1987, Cell.

[6]  N E Baker,et al.  Molecular cloning of sequences from wingless, a segment polarity gene in Drosophila: the spatial distribution of a transcript in embryos , 1987, The EMBO journal.

[7]  E. Wieschaus,et al.  Autonomous requirements for the segment polarity gene armadillo during Drosophila embryogenesis , 1987, Cell.

[8]  N. Perrimon,et al.  Multiple functions of segment polarity genes in Drosophila. , 1987, Developmental biology.

[9]  D L Brower,et al.  Engrailed gene expression in Drosophila imaginal discs. , 1986, The EMBO journal.

[10]  J. Lawrence,et al.  Intracellular localization of messenger RNAs for cytoskeletal proteins , 1986, Cell.

[11]  M. Noll,et al.  Isolation of the paired gene of Drosophila and its spatial expression during early embryogenesis , 1986, Nature.

[12]  P. Ingham,et al.  Transcription pattern of the Drosophila segmentation gene hairy , 1985, Nature.

[13]  P. O’Farrell,et al.  Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein , 1985, Cell.

[14]  R. Angerer,et al.  Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes. , 1984, Developmental biology.

[15]  H. Meinhardt.  Cell determination boundaries as organizing regions for secondary embryonic fields. , 1983, Developmental biology.

[16]  P. Lawrence.  The cellular basis of segmentation in insects , 1981, Cell.

[17]  P. Lawrence,et al.  Clonal analysis of the undifferentiated wing disk of Drosophila. , 1981, Developmental biology.

[18]  G. Struhl.  Anterior and posterior compartments in the proboscis of Drosophila. , 1981, Developmental biology.

[19]  Hans Meinhardt,et al.  Cooperation of Compartments for the Generation of Positional Information , 1980 .

[20]  C. Nüsslein-Volhard,et al.  Mutations affecting segment number and polarity in Drosophila , 1980, Nature.

[21]  P. Lawrence,et al.  The development of wingless, a homeotic mutation of Drosophila. , 1977, Developmental biology.

[22]  P. Lawrence,et al.  The early development of mesothoracic compartments in Drosophila. An analysis of cell lineage and fate mapping and an assessment of methods. , 1977, Developmental biology.

[23]  V. French,et al.  Pattern regulation in epimorphic fields. , 1976, Science.

[24]  E. Wieschaus,et al.  Clonal analysis of primordial disc cells in the early embryo of Drosophila melanogaster. , 1976, Developmental biology.

[25]  P. Lawrence,et al.  Compartments in the wing of Drosophila: a study of the engrailed gene. , 1976, Developmental biology.

[26]  V. Wigglesworth.  Evolution of Insect Wings and Flight , 1973, Nature.

[27]  A. Garcı́a-Bellido,et al.  Developmental analysis of the wing disc in the mutant engrailed of Drosophila melanogaster. , 1972, Genetics.

[28]  N. Baker.  Embryonic and imaginal requirements for wingless, a segment-polarity gene in Drosophila. , 1988, Developmental biology.

[29]  P. Lawrence,et al.  Borders of parasegments in Drosophila embryos are delimited by the fushi tarazu and even-skipped genes , 1987, Nature.

[30]  W. McGinnis,et al.  Isolation of a homoeo box-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts , 1985, Nature.

[31]  D. Lindsley,et al.  Genetic variations of Drosophila melanogaster , 1967 .