Effects of Water Stress, Defoliation and Crop Thinning on Vitis vinifera L. cv. Solaris Must and Wine Part II: 1H NMR Metabolomics

Proton nuclear magnetic resonance (1H NMR) metabolomics was employed to investigate the impact of water deficit, defoliation, and crop thinning on the chemical composition of must and wines from the cool-climate white grape variety Solaris. The obtained results show that viticultural practices (defoliation and crop thinning) affected the amino acid and sugar content of Solaris must and thereby the quality of the final wine—mainly in terms of compounds normally related to fruity aroma (i.e., isopentanol), non-sugar sweetness (i.e., proline and glycerol), and alcohol content. The content of tyrosol, a natural phenolic antioxidant with a high bioavailability, was increased in the final wine by a combination of defoliation and crop thinning. The results of the metabolomics analysis performed on the must and wine samples from the water stress experiment showed that short-term water deficit significantly affected the concentration of several flavor-related compounds, including glutamate, butyrate and propanol, of the organic acids lactate and fumarate, and of the phenolic compounds caffeic acid and p-coumaric acid. ANOVA simultaneous component analysis showed that the effect of water deficit accounted for 11% (p < 0.001) and 8% (p < 0.001) of the variability in the metabolite concentrations in must and wines, respectively, while viticultural practices accounted for 38% (p < 0.001) and 30% (p < 0.001) of the metabolite variability in must and wines, respectively.

[1]  C. Sgherri,et al.  Impact of Leaf Removal on Phenolics and Antioxidant Activity of Trebbiano Berries (Vitis vinifera L.) , 2022, Plants.

[2]  S. Engelsen,et al.  Effects of Water Stress, Defoliation and Crop Thinning on Vitis vinifera L. cv. Solaris: Part I: Plant Responses, Fruit Development and Fruit Quality , 2022, Metabolites.

[3]  G. Mattii,et al.  Effect of Agronomic Techniques on Aroma Composition of White Grapevines: A Review , 2021, Agronomy.

[4]  D. Osella,et al.  The effects of sulphur dioxide on wine metabolites: New insights from 1H NMR spectroscopy based in-situ screening, detection, identification and quantification , 2021 .

[5]  N. Esturau-Escofet,et al.  Comparison of two sample preparation methods for 1H-NMR wine profiling: Direct analysis and solid-phase extraction , 2021 .

[6]  J. Simal-Gándara,et al.  Bottle Aging and Storage of Wines: A Review , 2021, Molecules.

[7]  J. Riedl,et al.  NMR spectroscopy in wine authentication: An official control perspective. , 2021, Comprehensive reviews in food science and food safety.

[8]  B. Holzapfel,et al.  Sunburn in Grapes: A Review , 2021, Frontiers in Plant Science.

[9]  Jiang Zhu,et al.  Quality evaluation of Cabernet Sauvignon wines in different vintages by 1H nuclear magnetic resonance-based metabolomics , 2021 .

[10]  J. Simal-Gándara,et al.  Secondary Aroma: Influence of Wine Microorganisms in Their Aroma Profile , 2020, Foods.

[11]  S. Collin,et al.  Occurrence of Ehrlich-derived and Varietal Polyfunctional Thiols in Belgian White Wines made from Chardonnay and Solaris Grapes. , 2020, Journal of agricultural and food chemistry.

[12]  Jordi Ballester,et al.  Exploring the Typicality, Sensory Space, and Chemical Composition of Swedish Solaris Wines , 2020, Foods.

[13]  R. Larcher,et al.  Press Fractioning of Grape Juice: A First Step to Manage Potential Atypical Aging Development during Winemaking , 2020, American Journal of Enology and Viticulture.

[14]  D. Merdinoglu,et al.  Use of the NeoViGen96 chip to understand the defense status of cultivars and resistant genotypes of Vitis vinifera , 2019, Acta Horticulturae.

[15]  C. Pastore,et al.  Effects of Sunlight Exposure on Flavonol Content and Wine Sensory of the White Winegrape Grechetto Gentile , 2019, American Journal of Enology and Viticulture.

[16]  D. Rossouw,et al.  Organic Acid Metabolism and the Impact of Fermentation Practices on Wine Acidity: A Review , 2018, South African Journal of Enology and Viticulture.

[17]  J. Hernández-Hierro,et al.  Phenolic compounds and antioxidant activity of twelve grape cultivars measured by chemical and electrochemical methods , 2018, European Food Research and Technology.

[18]  R. Larcher,et al.  Shikimic acid concentration in white wines produced with different processing protocols from fungus-resistant grapes growing in the Alps , 2018 .

[19]  T. Toldam-Andersen,et al.  Cool-Climate Red Wines—Chemical Composition and Comparison of Two Protocols for 1H-NMR Analysis , 2018, Molecules.

[20]  R. Larcher,et al.  Targeted and untargeted high resolution mass approach for a putative profiling of glycosylated simple phenols in hybrid grapes. , 2017, Food research international.

[21]  R. Gonzalez,et al.  Wine secondary aroma: understanding yeast production of higher alcohols , 2017, Microbial biotechnology.

[22]  A. Sarkar,et al.  Oral tribology: Update on the relevance to study astringency in wines , 2017 .

[23]  Torben Bo Toldam-Andersen,et al.  Impact of sequential co-culture fermentations on flavour characters of Solaris wines , 2017, European Food Research and Technology.

[24]  A. Versari,et al.  Extraction and evaluation of natural occurring bioactive compounds and change in antioxidant activity during red winemaking , 2016, Journal of Food Science and Technology.

[25]  P. Darriet,et al.  The Impact of Climate Change on Viticulture and Wine Quality* , 2016, Journal of Wine Economics.

[26]  C. Skjöldebrand,et al.  The Nordic Light Terroir , 2016 .

[27]  M. Pérez-Coello,et al.  Wine science in the metabolomics era , 2015 .

[28]  M. Petersen,et al.  Influence of Pre-Fermentation Treatments on Wine Volatile and Sensory Profile of the New Disease Tolerant Cultivar Solaris , 2015, Molecules.

[29]  R. Töpfer,et al.  Sequence analysis of loci Rpv10 and Rpv3 for resistance against grapevine downy mildew ( Plasmopara viticola ) , 2014 .

[30]  T. Toldam-Andersen,et al.  Yeast species associated with different wine grape varieties in Denmark , 2013 .

[31]  Francesco Savorani,et al.  Investigations of La Rioja terroir for wine production using 1H NMR metabolomics. , 2012, Journal of agricultural and food chemistry.

[32]  Young-Shick Hong,et al.  NMR‐based metabolomics in wine science , 2011, Magnetic resonance in chemistry : MRC.

[33]  Florian F. Bauer,et al.  Wine flavor and aroma , 2011, Journal of Industrial Microbiology & Biotechnology.

[34]  Kashif Ali,et al.  Metabolic characterization of Palatinate German white wines according to sensory attributes, varieties, and vintages using NMR spectroscopy and multivariate data analyses , 2011, Journal of biomolecular NMR.

[35]  M. Pessarakli.  Role of Proline in Plant Response to Drought and Salinity , 2010 .

[36]  E. Kafkas,et al.  Distribution of major sugars, acids and total phenols in juice of five grapevine (Vitis spp.) cultivars at different stages of berry development. , 2010 .

[37]  S. Bastian,et al.  Sensory attributes of wine influenced by variety and berry shading discriminated by NMR metabolomics , 2010 .

[38]  F Savorani,et al.  icoshift: A versatile tool for the rapid alignment of 1D NMR spectra. , 2010, Journal of magnetic resonance.

[39]  A. Avenoza,et al.  A thorough study on the use of quantitative 1H NMR in Rioja red wine fermentation processes. , 2009, Journal of agricultural and food chemistry.

[40]  Won-Mok Park,et al.  Metabolomic studies on geographical grapes and their wines using 1H NMR analysis coupled with multivariate statistics. , 2009, Journal of agricultural and food chemistry.

[41]  J. Lindon,et al.  Systems biology: Metabonomics , 2008, Nature.

[42]  Mark Krstic,et al.  Cultural Practice and Environmental Impacts on the Flavonoid Composition of Grapes and Wine: A Review of Recent Research , 2006, American Journal of Enology and Viticulture.

[43]  A. Avenoza,et al.  Time course of the evolution of malic and lactic acids in the alcoholic and malolactic fermentation of grape must by quantitative 1H NMR (qHNMR) spectroscopy. , 2006, Journal of agricultural and food chemistry.

[44]  Age K. Smilde,et al.  ANOVA-simultaneous component analysis (ASCA): a new tool for analyzing designed metabolomics data , 2005, Bioinform..

[45]  Eveline J. Bartowsky,et al.  Yeast and bacterial modulation of wine aroma and flavour , 2005 .

[46]  P. Høj,et al.  Proline and arginine accumulation in developing berries of Vitis vinifera L. in Australian vineyards: influence of vine cultivar, berry maturity and tissue type. , 2000 .

[47]  D. J. Naylor,et al.  Proline accumulation in developing grapevine fruit occurs independently of changes in the levels of delta1-pyrroline-5-carboxylate synthetase mRNA or protein. , 1999, Plant physiology.

[48]  Paul Geladi,et al.  Principal Component Analysis , 1987, Comprehensive Chemometrics.

[49]  A. Reynolds,et al.  Viticultural and vineyard management practices and their effects on grape and wine quality , 2022, Managing Wine Quality.

[50]  M. Wolf,et al.  Evaluation of mechanical properties of berries on resistant or tolerant varieties of grapevine , 2019, BIO Web of Conferences.

[51]  M. Butnariu,et al.  Qualitative and Quantitative Chemical Composition of Wine , 2019, Quality Control in the Beverage Industry.

[52]  Jing Liu,et al.  Instrumental and sensory characterisation of Solaris white wines in Denmark. , 2015, Food chemistry.

[53]  G. Schernewski.  Adaptation to Climate Change: Viniculture and Tourism at the Baltic Coast , 2011 .

[54]  H. Kassemeyer,et al.  Different susceptibility of European grapevine cultivars for downy mildew , 2008 .

[55]  J. Huggett.  Geology and wine: a review , 2006 .

[56]  J. Marrugat,et al.  Bioavailability of tyrosol, an antioxidant phenolic compound present in wine and olive oil, in humans. , 2003, Drugs under experimental and clinical research.

[57]  Julie M. Tarara,et al.  Separation of Sunlight and Temperature Effects on the Composition of Vitis vinifera cv. Merlot Berries , 2002, American Journal of Enology and Viticulture.