Species-specificity of human antibeef, pork insulin serum.

Previous studies have revealed the presence of insulin-binding antibodies in the serums of insulin-treated human subjects. Antigen-antibody complexes in this system do not precipitate out of solution but are readily identified by paper chromato-electrophoresis employing insulin-I13' ( 1 ) . Kinetic studies of the reaction between crystalline beef insulin and antiserum have indicated that insulin is probably univalent and that there are at least two distinct orders of antibody-combining sites (2-4). Since insulins from five different mammalian species have been found to differ in amino acid sequence only in positions 8 to 10 of the A (glycyl) chain (5), it appeared of interest to study the reactivity and cross reactivity of these various insulins in the same antiserum. Studies with human insulin, several different preparations of beef insulin, including beef desamido insulin, and the A and B chains of insulin are also reported.

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