Supplementary effects of Saccharomyces boulardii and Bacillus subtilis B10 on digestive enzyme activities, antioxidation capacity and blood homeostasis in broiler.

The research was conducted to evaluate the feed supplementary effect of Saccharomyces boulardii and Bacillus subtilis on digestive enzyme activities, antioxidation capicity and blood homeostasis in broiler. A total of 300 day-old Sanhuang broilers (Chinese cross breed) were randomly divided into three groups with five replications in each group (n =20). The control group was fed basal diet contained antibiotic and experimental groups were supplemented with S. boulardii and B. subtilis in addition (1 × 10 8 cfu/kg) to basal diet for 72 days, respectively. The results revealed that activities of jejunal Na + -K + ATPase (ATP), lipase (LP) and Gamma glutamyl transpeptidase (γGT) concentration increased (P<0.05) in probiotic supplementary groups. Moreover, results from ileum showed high levels of γGT in B. subtilis supplemented group only. The serum glutathione peroxidase (GSH-PX), peroxidase (POD), glutathione (GSH), glutathione reductase (GR) and catalase (CAT) concentration were significantly higher, while there was a significant decrease (P <0.05) in malondialdehyde (MDA) content in supplementary groups. The blood biochemical analysis showed significant decrease in uric acid and triglycerides of S. boulardii and B. subtilis groups. Conversely, albumin and low-density lipoprotein concentration increased in S. boulardii and B. subtilis groups as compare to control. The present research revealed that, supplementation of S. boulardii and B. subtilis B10 could be applied to enhance digestive enzyme activities, antioxidation and blood profile of broilers. In addition it might be considered as a natural antioxidant feed additive for broiler. © 2013 Friends Science Publishers

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