TaSINA2B, interacting with TaSINA1D, positively regulates drought tolerance and root growth in wheat (Triticum aestivum L.).

Wheat (Triticum aestivum L.) is an important food crop mainly grown in arid and semiarid regions worldwide, whose productivity is severely limited by drought stress. Although various E3 ubiquitin (Ub) ligases regulate drought stress, only a few SINA-type E3 Ub ligases are known to participate in such responses. Herein, we identified and cloned 15 TaSINAs from wheat. The transcription level of TaSINA2B was highly induced by drought, osmotic and abscisic acid treatments. Two-type promoters of TaSINA2B were found in 192 wheat accessions; furthermore wheat accessions with promoter TaSINA2BII showed a considerably higher level of drought tolerance and gene expression levels than those characterizing accessions with promoter TaSINA2BI that was mainly caused by a 64 bp insertion in its promoter. Enhanced drought tolerance of TaSINA2B-overexpressing (OE) transgenic wheat lines was found to be associated with root growth promotion. Further, an interaction between TaSINA2B and TaSINA1D was detected through yeast two-hybrid and bimolecular fluorescence complementation assays. And TaSINA1D-OE transgenic wheat lines showed similar drought tolerance and root growth phenotype to those observed when TaSINA2B was overexpressed. Therefore, the variation of TaSINA2B promoter contributed to the drought stress regulation, while TaSINA2B, interacting with TaSINA1D, positively regulated drought tolerance by promoting root growth.

[1]  Bao Liu,et al.  Wheat genomic study for genetic improvement of traits in China , 2022, Science China Life Sciences.

[2]  Bin Chen,et al.  Genome-wide association study revealed TaHXK3-2A as a candidate gene controlling stomatal index in wheat seedlings. , 2022, Plant, cell & environment.

[3]  Tian Li,et al.  TaGW2L, a GW2-like RING finger E3 ligase, positively regulates heading date in common wheat (Triticum aestivum L.) , 2022, The Crop Journal.

[4]  Lina Jiang,et al.  A NAC transcription factor, TaNAC5D-2, acts as a positive regulator of drought tolerance through regulating water loss in wheat (Triticum aestivum L.) , 2022, Environmental and Experimental Botany.

[5]  Z. Kang,et al.  Variation in cis-Regulation of a NAC Transcription Factor Contributes to Drought Tolerance in Wheat. , 2021, Molecular plant.

[6]  Wei Li,et al.  SEVEN IN ABSENTIA Ubiquitin Ligases Positively Regulate Defense Against Verticillium dahliae in Gossypium hirsutum , 2021, Frontiers in Plant Science.

[7]  J. Rogers,et al.  Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly , 2021, The Plant journal : for cell and molecular biology.

[8]  Sudhir Kumar,et al.  MEGA11: Molecular Evolutionary Genetics Analysis Version 11 , 2021, Molecular biology and evolution.

[9]  Chaonan Li,et al.  Recognizing the hidden half in wheat: root system attributes associated with drought tolerance. , 2021, Journal of experimental botany.

[10]  Chao Yang,et al.  SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants. , 2020, Journal of integrative plant biology.

[11]  Liwen Jiang,et al.  SINAT E3 Ubiquitin Ligases Mediate FREE1 and VPS23A Degradation to Modulate Abscisic Acid Signaling , 2020, Plant Cell.

[12]  C. You,et al.  Genome-Wide Identification of Apple Ubiquitin SINA E3 Ligase and Functional Characterization of MdSINA2 , 2020, Frontiers in Plant Science.

[13]  Liqun Li,et al.  Wheat RING E3 ubiquitin ligase TaDIS1 degrade TaSTP via the 26S proteasome pathway. , 2020, Plant science : an international journal of experimental plant biology.

[14]  Z. Kang,et al.  Regulatory changes in TaSNAC8‐6A are associated with drought tolerance in wheat seedlings , 2019, Plant biotechnology journal.

[15]  D. An,et al.  TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.) , 2019, Plant biotechnology journal.

[16]  Liang Yang,et al.  Genome-Wide Identification, Evolution, and Expression Analysis of RING Finger Gene Family in Solanum lycopersicum , 2019, International journal of molecular sciences.

[17]  Ari Pekka Mähönen,et al.  Transcriptional regulatory framework for vascular cambium development in Arabidopsis roots , 2019, Nature Plants.

[18]  Guo‐Liang Wang,et al.  SINA E3 Ubiquitin Ligases: Versatile Moderators of Plant Growth and Stress Response. , 2019, Molecular plant.

[19]  Liqun Li,et al.  Isolation and identification of wheat gene TaDIS1 encoding a RING finger domain protein, which negatively regulates drought stress tolerance in transgenic Arabidopsis. , 2018, Plant science : an international journal of experimental plant biology.

[20]  Sung Chul Lee,et al.  Roles of pepper bZIP protein CaDILZ1 and its interacting partner RING-type E3 ligase CaDSR1 in modulation of drought tolerance. , 2018, The Plant journal : for cell and molecular biology.

[21]  Jonathan D. G. Jones,et al.  Shifting the limits in wheat research and breeding using a fully annotated reference genome , 2018, Science.

[22]  P. Khurana,et al.  Gene encoding vesicle-associated membrane protein-associated protein from Triticum aestivum (TaVAP) confers tolerance to drought stress , 2017, Cell Stress and Chaperones.

[23]  Yongsheng Liu,et al.  Functional analysis of the seven in absentia ubiquitin ligase family in tomato. , 2018, Plant, cell & environment.

[24]  Lina Jiang,et al.  Overexpression of TaWRKY146 Increases Drought Tolerance through Inducing Stomatal Closure in Arabidopsis thaliana , 2017, Front. Plant Sci..

[25]  Wenyu Yang,et al.  E3 Ubiquitin Ligases: Ubiquitous Actors in Plant Development and Abiotic Stress Responses. , 2017, Plant & cell physiology.

[26]  Trevor M. Nolan,et al.  SINAT E3 Ligases Control the Light-Mediated Stability of the Brassinosteroid-Activated Transcription Factor BES1 in Arabidopsis. , 2017, Developmental cell.

[27]  L. Bögre,et al.  The low oxygen, oxidative and osmotic stress responses synergistically act through the ethylene response factor VII genes RAP2.12, RAP2.2 and RAP2.3. , 2015, The Plant journal : for cell and molecular biology.

[28]  Yanpeng Wang,et al.  Genome editing in rice and wheat using the CRISPR/Cas system , 2014, Nature Protocols.

[29]  H. Liu,et al.  The tumor necrosis factor receptor-associated factor (TRAF)-like family protein SEVEN IN ABSENTIA 2 (SINA2) promotes drought tolerance in an ABA-dependent manner in Arabidopsis. , 2014, The New phytologist.

[30]  J. von Braun,et al.  Climate Change Impacts on Global Food Security , 2013, Science.

[31]  J. Foley,et al.  Yield Trends Are Insufficient to Double Global Crop Production by 2050 , 2013, PloS one.

[32]  S. Yoshida,et al.  Lotus japonicus E3 Ligase SEVEN IN ABSENTIA4 Destabilizes the Symbiosis Receptor-Like Kinase SYMRK and Negatively Regulates Rhizobial Infection[C][W] , 2012, Plant Cell.

[33]  Qingzhen Zhao,et al.  The SINA E3 Ligase OsDIS1 Negatively Regulates Drought Response in Rice1[C][W][OA] , 2011, Plant Physiology.

[34]  Satoshi Tabata,et al.  Conservation of Lotus and Arabidopsis Basic Helix-Loop-Helix Proteins Reveals New Players in Root Hair Development1[W][OA] , 2009, Plant Physiology.

[35]  R. Deshaies,et al.  RING domain E3 ubiquitin ligases. , 2009, Annual review of biochemistry.

[36]  Peer Bork,et al.  SMART 6: recent updates and new developments , 2008, Nucleic Acids Res..

[37]  S. Rombauts,et al.  Seven in Absentia Proteins Affect Plant Growth and Nodulation in Medicago truncatula1[W][OA] , 2008, Plant Physiology.

[38]  Paul Linstead,et al.  An Ancient Mechanism Controls the Development of Cells with a Rooting Function in Land Plants , 2007, Science.

[39]  N. Varin‐Blank,et al.  Dimerization of hSiah proteins regulates their stability. , 2006, Biochemical and biophysical research communications.

[40]  S. Yang,et al.  Upregulation of an Arabidopsis RING-H2 gene, XERICO, confers drought tolerance through increased abscisic acid biosynthesis. , 2006, The Plant journal : for cell and molecular biology.

[41]  Robert D. Finn,et al.  Pfam: clans, web tools and services , 2005, Nucleic Acids Res..

[42]  R. Vierstra,et al.  The ubiquitin 26S proteasome proteolytic pathway. , 2004, Annual review of plant biology.

[43]  C. Bernhardt,et al.  The bHLH genes GLABRA3 (GL3) andENHANCER OF GLABRA3 (EGL3) specify epidermal cell fate in the Arabidopsis root , 2003, Development.

[44]  R. Stupar,et al.  The HECT ubiquitin-protein ligase (UPL) family in Arabidopsis: UPL3 has a specific role in trichome development. , 2003, The Plant journal : for cell and molecular biology.

[45]  N. Chua,et al.  SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signals , 2002, Nature.

[46]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[47]  E. Fearon,et al.  Siah-1 N-Terminal RING Domain Is Required for Proteolysis Function, and C-Terminal Sequences Regulate Oligomerization and Binding to Target Proteins , 1999, Molecular and Cellular Biology.

[48]  Y. Li,et al.  Photoreceptor Cell Differentiation Requires Regulated Proteolysis of the Transcriptional Repressor Tramtrack , 1997, Cell.

[49]  G. Rubin,et al.  seven in absentia, a gene required for specification of R7 cell fate in the Drosophila eye , 1990, Cell.