A targeted therapy for melanoma by graphene oxide composite with microRNA carrier

Background Nowadays, the combination of microRNA (miR) is attracting increased attention in clinical cancer trials. However, the clinical use of miR is highly limited because of certain properties such as instability, low-specificity distribution, and metabolic toxicity. Methods In order to improve the anti-tumor efficacy and reduce the side effects of miR in treating melanoma, a combination of graphene oxide (GO), chitosan (CS), and a cellular penetrating peptide, MPG, was prepared with solid dispersion method in this research. The research has analyzed the specific components of nano drug-loading complexes GO-CS and GO-CS-MPG through characterization research and confirmed the bio-safety of the carrier material GO-CS-MPG. Results The GO-CS-MPG-miR33a/miR199a nano drug-loading complex was successfully constructed and its medical effectiveness was verified. Through the subcutaneous tumor implantation experiment, an evident effect of the drug-loading complex in inhibiting melanoma cells was proven. Conclusion Results suggest that GO-CS-MPG may have potential applications in melanoma therapy.

[1]  S. Hamm-Alvarez,et al.  Current status of gene delivery and gene therapy in lacrimal gland using viral vectors. , 2006, Advanced drug delivery reviews.

[2]  L. Chaloin,et al.  A new peptide vector for efficient delivery of oligonucleotides into mammalian cells. , 1997, Nucleic acids research.

[3]  V. Sanna,et al.  Targeted therapy using nanotechnology: focus on cancer , 2014, International journal of nanomedicine.

[4]  Jianda Zhou,et al.  miR-33a functions as a tumor suppressor in melanoma by targeting HIF-1α , 2015, Cancer biology & therapy.

[5]  W. Xiong,et al.  miR-199a-5p regulates the expression of metastasis-associated genes in B16F10 melanoma cells. , 2014, International Journal of Clinical and Experimental Pathology.

[6]  L. Cantley,et al.  Understanding the Warburg Effect: The Metabolic Requirements of Cell Proliferation , 2009, Science.

[7]  T. Ochiya,et al.  RNAi therapeutics and applications of microRNAs in cancer treatment. , 2013, Japanese journal of clinical oncology.

[8]  Martin L Read,et al.  Delivery of siRNA mediated by histidine-containing reducible polycations. , 2008, Journal of controlled release : official journal of the Controlled Release Society.

[9]  Yi-Zeng Liang,et al.  Classification of vinegar samples based on near infrared spectroscopy combined with wavelength selection , 2011 .

[10]  Ming Zhou,et al.  Let-7b and microRNA-199a inhibit the proliferation of B16F10 melanoma cells. , 2012, Oncology letters.

[11]  H. Edwards,et al.  Combined FT–Raman spectroscopic and mass spectrometric study of ancient Egyptian sarcophagal fragments , 2007, Analytical and bioanalytical chemistry.

[12]  Azam Bolhassani,et al.  Potential efficacy of cell-penetrating peptides for nucleic acid and drug delivery in cancer. , 2011, Biochimica et biophysica acta.

[13]  Xueguang Shao,et al.  A wavelength selection method based on randomization test for near-infrared spectral analysis , 2009 .

[14]  Eun-Kyung Lim,et al.  Delivery of Cancer Therapeutics Using Nanotechnology , 2013, Pharmaceutics.

[15]  Xueguang Shao,et al.  Application of latent projective graph in variable selection for near infrared spectral analysis , 2012 .

[16]  Ming Zhou,et al.  Identification of FLOT2 as a novel target for microRNA-34a in melanoma , 2015, Journal of Cancer Research and Clinical Oncology.

[17]  Khudzir Ismail,et al.  Combustion characteristics of Malaysian oil palm biomass, sub-bituminous coal and their respective blends via thermogravimetric analysis (TGA). , 2012, Bioresource technology.

[18]  Zhiping Zhang,et al.  Lipid-enveloped hybrid nanoparticles for drug delivery. , 2013, Nanoscale.

[19]  D. Rigel,et al.  The Evolution of Melanoma Diagnosis: 25 Years Beyond the ABCDs , 2010, CA: a cancer journal for clinicians.

[20]  Jintian Tang,et al.  Analysis of lncRNAs expression in UVB-induced stress responses of melanocytes. , 2016, Journal of dermatological science.

[21]  Bulent Ozpolat,et al.  Liposomal siRNA nanocarriers for cancer therapy. , 2014, Advanced drug delivery reviews.

[22]  Xiaoyang Xu,et al.  Cancer nanotechnology: the impact of passive and active targeting in the era of modern cancer biology. , 2014, Advanced drug delivery reviews.

[23]  D. Hanahan,et al.  Hallmarks of Cancer: The Next Generation , 2011, Cell.

[24]  W. Cai,et al.  Variable selection based on locally linear embedding mapping for near-infrared spectral analysis , 2014 .

[25]  Ronit Satchi-Fainaro,et al.  Nano-sized polymers and liposomes designed to deliver combination therapy for cancer. , 2013, Current opinion in biotechnology.