Natural ingredient paeoniflorin could be a lead compound against white spot syndrome virus infection in Litopenaeus vannamei.

White spot syndrome virus (WSSV) is an important pathogen causing high mortality in the shrimp industry in aquaculture, yet there is no treatment available to date. In order to find a treatment against WSSV infection, this study examined the anti-WSSV activity of eight natural compounds using shrimp larvae as a model. Among the eight compounds, paeoniflorin showed the most obvious anti-WSSV effect, with a maximum protection efficiency of WSSV-infected shrimp >60% at 100 μM. Furthermore, pretreatment and post-treatment experiments revealed that paeoniflorin could prevent and treat WSSV infection in shrimp. The antiviral activity of paeoniflorin in aquaculture water decreased rapidly with time, and the results showed that the stable anti-WSSV activity of paeoniflorin could only remain in water for 1 day. Thus, the dosing pattern of continuous medication changes was evaluated. Obviously, in the model of continuous change of paeoniflorin, WSSV copy numbers in the virus-treated shrimp group still progressively increased, while the virus content in WSSVpaeoniflorin -treated group continued to decrease. Interestingly, paeoniflorin inhibited horizontal transmission of WSSV to a certain extent. Notably, paeoniflorin significantly increased the expression of antimicrobial peptides of shrimp to resist WSSV. In conclusion, paeoniflorin has the potential to protect shrimp against WSSV.

[1]  M. Yan,et al.  Evaluation on the antiviral activity of a nitrile phenylpropanoid against white spot syndrome virus infection in Litopenaeus vannamei , 2022, Aquaculture.

[2]  Lei Liu,et al.  Small Molecule Inhibitors of White Spot Syndrome Virus: Promise in Shrimp Seedling Culture , 2021, International journal of molecular sciences.

[3]  Jian-Ping Chen,et al.  Difference in medication pattern potentially enhances antiviral efficiency of a novel amino-fluorophenyl compound on WSS risk in shrimp seedling culture , 2021 .

[4]  M. Yan,et al.  A novel antiviral coumarin derivative as a potential agent against WSSV infection in shrimp seedling culture. , 2021, Virus research.

[5]  Yanhe Li,et al.  The SNP polymorphisms associated with WSSV-resistance of prophenoloxidase in red swamp crayfish (Procambarus clarkii) and its immune response against white spot syndrome virus (WSSV) , 2021 .

[6]  M. Yan,et al.  Infection of WSSV shows potential promise of a novel antiviral amino-nitrophenyl medicine for application in culture of shrimp seedling , 2020 .

[7]  Xiaozhou Qi,et al.  Antiviral activity of geniposidic acid against white spot syndrome virus replication in red swamp crayfish Procambarus clarkii , 2020 .

[8]  Wen-Ju Wang,et al.  Antidepressant Effect of Paeoniflorin Is Through Inhibiting Pyroptosis CASP-11/GSDMD Pathway , 2020, Molecular neurobiology.

[9]  Gaoxue Wang,et al.  Ursolic acid from Prunella vulgaris L. efficiently inhibits IHNV infection in vito and in vivo. , 2019, Virus research.

[10]  Gaoxue Wang,et al.  Evaluation on the antiviral activity of genipin against white spot syndrome virus in crayfish. , 2019, Fish & shellfish immunology.

[11]  Gaoxue Wang,et al.  Saikosaponin D efficiently inhibits SVCV infection in vitro and in vivo , 2019, Aquaculture.

[12]  Xiaozhou Qi,et al.  Gardenia jasminoides Ellis inhibit white spot syndrome virus replication in red swamp crayfish Procambarus clarkii , 2019, Aquaculture.

[13]  Jianguo He,et al.  RNAi screening identifies a new Toll from shrimp that restricts WSSV infection through activating Dorsal to induce antimicrobial peptides , 2018, bioRxiv.

[14]  Rongning Liang,et al.  PI3K signaling pathways modulated white spot syndrome virus (WSSV) replication in Procambarus clarkii , 2018, Fish & shellfish immunology.

[15]  Gaoxue Wang,et al.  Evaluation on the antiviral activity of arctigenin against spring viraemia of carp virus , 2017, Aquaculture.

[16]  Benhur Lee,et al.  Inhibition of an Aquatic Rhabdovirus Demonstrates Promise of a Broad-Spectrum Antiviral for Use in Aquaculture , 2016, Journal of Virology.

[17]  Hai-Peng Liu,et al.  Identification of an anti-lipopolysacchride factor possessing both antiviral and antibacterial activity from the red claw crayfish Cherax quadricarinatus. , 2016, Fish & shellfish immunology.

[18]  K. Hartshorn,et al.  The Role of Antimicrobial Peptides in Influenza Virus Infection and Their Potential as Antiviral and Immunomodulatory Therapy , 2016, Pharmaceuticals.

[19]  Grant D. Stentiford,et al.  Molecular Mechanisms of White Spot Syndrome Virus Infection and Perspectives on Treatments , 2016, Viruses.

[20]  J. Vlak,et al.  Horizontal transmission dynamics of White spot syndrome virus by cohabitation trials in juvenile Penaeus monodon and P. vannamei. , 2014, Preventive veterinary medicine.

[21]  C. Lo,et al.  Anti-lipopolysaccharide factor isoform 3 from Penaeus monodon (ALFPm3) exhibits antiviral activity by interacting with WSSV structural proteins. , 2014, Antiviral research.

[22]  I. Karunasagar,et al.  Biology, Host Range, Pathogenesis and Diagnosis of White spot syndrome virus , 2012, Indian Journal of Virology.

[23]  K. Tang,et al.  New genotypes of white spot syndrome virus (WSSV) and Taura syndrome virus (TSV) from the Kingdom of Saudi Arabia. , 2012, Diseases of aquatic organisms.

[24]  E. A. Tendencia,et al.  Temperature fluctuation, low salinity, water microflora: Risk factors for WSSV outbreaks in Penaeus monodon , 2011 .

[25]  Yi-Da Wang,et al.  Antiviral activity by fish antimicrobial peptides of epinecidin-1 and hepcidin 1–5 against nervous necrosis virus in medaka , 2010, Peptides.

[26]  S. Chi,et al.  Antimicrobial peptides (AMP) with antiviral activity against fish nodavirus. , 2010, Fish & shellfish immunology.

[27]  A. Sánchez‐Paz.  White spot syndrome virus: an overview on an emergent concern , 2010, Veterinary research.

[28]  C. Lo,et al.  Whispovirus. , 2022, Current topics in microbiology and immunology.

[29]  Yun‐wei Dong,et al.  Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission , 2009, Veterinary Research Communications.

[30]  I. Hirono,et al.  Essential function of transglutaminase and clotting protein in shrimp immunity. , 2008, Molecular immunology.

[31]  G. Bovo,et al.  Viral disease of cultured marine fish. , 2008 .

[32]  W. Wei,et al.  Paeoniflorin suppresses inflammatory mediator production and regulates G protein-coupled signaling in fibroblast – like synoviocytes of collagen induced arthritic rats , 2008, Inflammation Research.

[33]  L. Cerenius,et al.  Antilipopolysaccharide Factor Interferes with White Spot Syndrome Virus Replication In Vitro and In Vivo in the Crayfish Pacifastacus leniusculus , 2006, Journal of Virology.

[34]  K. Söderhäll,et al.  Cell-mediated immunity in arthropods: hematopoiesis, coagulation, melanization and opsonization. , 2006, Immunobiology.

[35]  Wei-yu Zhang,et al.  Paeoniflorin attenuates neuroinflammation and dopaminergic neurodegeneration in the MPTP model of Parkinson's disease by activation of adenosine A1 receptor , 2006, British journal of pharmacology.

[36]  Feng Yang,et al.  White spot syndrome virus (WSSV) infectivity for Artemia at different developmental stages. , 2003, Diseases of aquatic organisms.

[37]  D. Lightner,et al.  Quantitative real time PCR for the measurement of white spot syndrome virus in shrimp , 2002 .

[38]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[39]  G. Brennan,et al.  White spot baculovirus syndrome in the Indian shrimp Penaeus monodon and P. indicus. , 2000 .

[40]  C. Lo,et al.  Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization , 1996 .

[41]  C. Lo,et al.  Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan , 1995 .