The effect of microdialysis needle trauma on cutaneous vascular responses in humans.

Microdialysis enables in-depth mechanistic study of the cutaneous circulation in humans. However, whether the insertion or presence of the microdialysis fiber (MDF) affects the skin circulation or its responses is unknown. We tested whether the cutaneous vascular response to whole body heating (WBH) was affected by MDF or by pretreatment with ice (part 1) or local anesthesia (LA; part 2). Eleven subjects participated, 9 in part 1 and 8 in part 2 (5 participated in both). In both parts, four sites on the forearm were selected, providing untreated control, MDF only, ice or LA only, and combined MDF plus ice or LA. A tube-lined suit controlled whole body skin temperature, which was raised to approximately 38 degrees C for WBH. Skin sites were instrumented with laser-Doppler flow probes. Data were expressed as cutaneous vascular conductance (CVC). Baseline levels were not different among sites (P > 0.05). In part 1, the internal temperature for the onset of vasodilation was higher (P > 0.05) with MDF with or without ice pretreatment than at untreated control sites (control 36.6 +/- 0.1 degrees C, Ice 36.5 +/- 0.1, MDF 36.8 +/- 0.1 degrees C, and Ice+MDF 36.8 +/- 0.1 degrees C). Peak CVC during WBH was decreased (P < 0.05) by MDF (control 73 +/- 7 vs. MDF 59 +/- 6% of maximal CVC). Ice (73 +/- 6% of maximal CVC) or Ice+MDF (69 +/- 6% of maximal CVC) did not affect (P > 0.05) peak CVC compared with control. In part 2, the temperature threshold for the onset of vasodilation was increased by MDF with or without LA treatment and by LA alone (P < 0.05; control 36.6 +/- 0.1 degrees C, MDF 36.7 +/- 0.1 degrees C, LA 36.8 +/- 0.1 degrees C, and LA+MDF 36.8 +/- 0.1 degrees C). Peak CVC was decreased by MDF (control 69 +/- 6% of maximal CVC vs. MDF 58 +/- 8% of maximal CVC; P < 0.05). LA only (65 +/- 10% of maximal CVC) or MDF in the presence of LA (73 +/- 12% of maximal CVC) did not affect (P > 0.05) peak CVC compared with control. Thus LA or MDF increases the temperature threshold for the onset of vasodilation. MDF alone decreases the peak vasodilator response in CVC to WBH; however, this attenuation did not occur if ice or LA is used before MDF placement. Ice or LA alone do not affect the peak response in CVC to WBH. How those treatments prevent or reverse the effect of MDF placement is presently unclear.

[1]  A. P. Shepherd,et al.  Laser-Doppler Blood Flowmetry , 2010, Developments in Cardiovascular Medicine.

[2]  G. Hodges,et al.  The involvement of norepinephrine, neuropeptide Y, and nitric oxide in the cutaneous vasodilator response to local heating in humans. , 2008, Journal of applied physiology.

[3]  D. Kellogg,et al.  Neuronal nitric oxide synthase control mechanisms in the cutaneous vasculature of humans in vivo , 2008, The Journal of physiology.

[4]  G. Hodges,et al.  The role of baseline in the cutaneous vasoconstrictor responses during combined local and whole body cooling in humans. , 2007, American journal of physiology. Heart and circulatory physiology.

[5]  C. Minson,et al.  Cutaneous neuronal nitric oxide is specifically decreased in postural tachycardia syndrome. , 2007, American journal of physiology. Heart and circulatory physiology.

[6]  Kun Zhao,et al.  The role of sensory nerves in the cutaneous vasoconstrictor response to local cooling in humans , 2007, American journal of physiology. Heart and circulatory physiology.

[7]  J. Cracowski,et al.  Effects of local anaesthesia on subdermal needle insertion pain and subsequent tests of microvascular function in human. , 2007, European journal of pharmacology.

[8]  C. Minson,et al.  Neurokinin‐1 receptor desensitization attenuates cutaneous active vasodilatation in humans , 2006, The Journal of physiology.

[9]  G. Hodges,et al.  The involvement of nitric oxide in the cutaneous vasoconstrictor response to local cooling in humans , 2006, The Journal of physiology.

[10]  John M. Johnson,et al.  Relative roles of local and reflex components in cutaneous vasoconstriction during skin cooling in humans. , 2006, Journal of applied physiology.

[11]  N. Tublitz,et al.  Neurokinin‐1 receptor desensitization to consecutive microdialysis infusions of substance P in human skin , 2005, The Journal of physiology.

[12]  Lacy A. Holowatz,et al.  Cutaneous vasoconstrictor responses to norepinephrine are attenuated in older humans. , 2005, American journal of physiology. Regulatory, integrative and comparative physiology.

[13]  John M. Johnson,et al.  Sympathetic, sensory, and nonneuronal contributions to the cutaneous vasoconstrictor response to local cooling. , 2005, American journal of physiology. Heart and circulatory physiology.

[14]  C. Minson,et al.  H1 but not H2 histamine receptor activation contributes to the rise in skin blood flow during whole body heating in humans , 2004, The Journal of physiology.

[15]  N. Tublitz,et al.  Mechanisms of vasoactive intestinal peptide-mediated vasodilation in human skin. , 2004, Journal of applied physiology.

[16]  John M. Johnson,et al.  Neuropeptide Y antagonism reduces reflex cutaneous vasoconstriction in humans. , 2004, American journal of physiology. Heart and circulatory physiology.

[17]  Caitlin Thompson,et al.  Altered neurotransmitter control of reflex vasoconstriction in aged human skin , 2004, The Journal of physiology.

[18]  John M. Johnson,et al.  Evidence for a Role for Vasoactive Intestinal Peptide in Active Vasodilatation in the Cutaneous Vasculature of Humans , 2003, The Journal of physiology.

[19]  C. Minson,et al.  Nitric oxide is not permissive for cutaneous active vasodilatation in humans , 2003, The Journal of physiology.

[20]  L. Roman,et al.  Nitric oxide concentration increases in the cutaneous interstitial space during heat stress in humans. , 2003, Journal of applied physiology.

[21]  U. Wahn,et al.  Down-regulation of vasoactive intestinal polypeptide receptor expression in atopic dermatitis. , 2003, The Journal of allergy and clinical immunology.

[22]  M. Schmelz,et al.  The Effect of the Nitric Oxide Synthase Inhibitor N-Nitro-L-Arginine-Methyl Ester on Neuropeptide-Induced Vasodilation and Protein Extravasation in Human Skin , 2003, Journal of Vascular Research.

[23]  A. Hancock,et al.  Differential Activation of Dual Signaling Responses by Human H1 and H2 Histamine Receptors , 2003, Journal of receptor and signal transduction research.

[24]  W. L. Kenney,et al.  Decreased nitric oxide- and axon reflex-mediated cutaneous vasodilation with age during local heating. , 2002, Journal of applied physiology.

[25]  G. Clough,et al.  Vascular responses in the skin: an accessible model of inflammation. , 2002, News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society.

[26]  D. Groneberg,et al.  Simultaneous detection of receptor mRNA and ligand protein in human skin tissues , 2002, Journal of cutaneous pathology.

[27]  M. Joyner,et al.  Nitric oxide and neurally mediated regulation of skin blood flow during local heating. , 2001, Journal of applied physiology.

[28]  J. Huidobro-Toro,et al.  Molecular mechanism of cGMP‐mediated smooth muscle relaxation , 2000, Journal of cellular physiology.

[29]  R. Lefebvre,et al.  Interplay between nitric oxide and vasoactive intestinal polypeptide in the pig gastric fundus smooth muscle. , 2000, European journal of pharmacology.

[30]  M. Joyner,et al.  Effects of atropine and L-NAME on cutaneous blood flow during body heating in humans. , 2000, Journal of applied physiology.

[31]  W. Kirch,et al.  Histamine response and local cooling in the human skin: involvement of H1‐ and H2‐receptors , 1999 .

[32]  J. Johnson,et al.  Reflex control of cutaneous vasoconstrictor system is reset by exogenous female reproductive hormones. , 1999, Journal of applied physiology.

[33]  D L Kellogg,et al.  Role of nitric oxide in the vascular effects of local warming of the skin in humans. , 1999, Journal of applied physiology.

[34]  G. Clough.  Role of nitric oxide in the regulation of microvascular perfusion in human skin in vivo , 1999, The Journal of physiology.

[35]  L. Petersen,et al.  Pituitary adenylate cyclase activating polypeptide (PACAP) is localized in human dermal neurons and causes histamine release from skin mast cells , 1998, Inflammation Research.

[36]  C. Crandall,et al.  Nitric oxide and cutaneous active vasodilation during heat stress in humans. , 1998, Journal of applied physiology.

[37]  J. Serup,et al.  Cutaneous Microdialysis in the Rat: Insertion Trauma and Effect of Anaesthesia Studied by Laser Doppler Perfusion Imaging and Histamine Release , 1998, Skin Pharmacology and Physiology.

[38]  M. Church,et al.  Measurement of nitric oxide concentration in human skin in vivo using dermal microdialysis , 1998, Experimental physiology.

[39]  R. Rukwied,et al.  Cutaneous reactions and sensations after intracutaneous injection of vasoactive intestinal polypeptide and acetylcholine in atopic eczema patients and healthy controls , 1998, Archives of Dermatological Research.

[40]  C. Estrada,et al.  Neuronal Nitric Oxide Synthase Activation by Vasoactive Intestinal Peptide in Bovine Cerebral Arteries , 1997, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[41]  R. Etzel,et al.  Evidence of functional beta-adrenoceptors in the cutaneous vasculature. , 1997, The American journal of physiology.

[42]  R. Funk,et al.  Immunohistochemical detection of human skin nerve fibers. , 1997, Acta Histochemica.

[43]  R. Etzel,et al.  EVIDENCE OF FUNCTIONAL ??-ADRENOCEPTORS IN THE CUTANEOUS VASCULATURE771 , 1997 .

[44]  J. Olesen,et al.  Role of endothelium and nitric oxide in histamine‐induced responses in human cranial arteries and detection of mRNA encoding H1‐ and H2‐receptors by RT‐PCR , 1997, British journal of pharmacology.

[45]  V. S. Bishop,et al.  The roles of cGMP and cAMP in active thermoregulatory vasodilation. , 1997, The American journal of physiology.

[46]  S. Kudoh,et al.  Synergistic effect of nitric oxide and vasoactive intestinal peptide on bronchoprotection against histamine in anesthetized guinea pigs. , 1997, American journal of respiratory and critical care medicine.

[47]  G. Makhlouf,et al.  Stoichiometry of neurally induced VIP release, NO formation, and relaxation in rabbit and rat gastric muscle. , 1996, The American journal of physiology.

[48]  L. Ackermann,et al.  Neuropeptide- and capsaicin-induced histamine release in skin monitored with the microdialysis technique. , 1996, Acta dermato-venereologica.

[49]  J. Cohen,et al.  Neuropeptides induce release of nitric oxide from human dermal microvascular endothelial cells. , 1996, The Journal of investigative dermatology.

[50]  M. Jamieson,et al.  The effect of iontophoresis on the cutaneous vasculature: evidence for current-induced hyperemia. , 1995, Microvascular research.

[51]  K. Wårdell,et al.  Changes in skin circulation after insertion of a microdialysis probe visualized by laser Doppler perfusion imaging. , 1994, The Journal of investigative dermatology.

[52]  J. Grider.  Interplay of VIP and nitric oxide in regulation of the descending relaxation phase of peristalsis. , 1993, The American journal of physiology.

[53]  G. Makhlouf,et al.  Stimulation of nitric oxide from muscle cells by VIP: prejunctional enhancement of VIP release. , 1992, The American journal of physiology.

[54]  G. Brengelmann,et al.  Cystic fibrosis, vasoactive intestinal polypeptide, and active cutaneous vasodilation. , 1990, Journal of applied physiology.

[55]  R. Geronemus,et al.  Effect of the topical anesthetic EMLA on the efficacy of pulsed dye laser treatment of port-wine stains. , 1990, The Journal of dermatologic surgery and oncology.

[56]  W. Kummer,et al.  Vasoactive intestinal polypeptide receptor-like immunoreactivity in human sweat glands , 1990, Neuroscience Letters.

[57]  G. Parati,et al.  Comparison of Finger and Intra‐arterial Blood Pressure Monitoring at Rest and During Laboratory Testing , 1989, Hypertension.

[58]  J. Johnson,et al.  Cutaneous vascular responses to isometric handgrip exercise. , 1989, Journal of applied physiology.

[59]  M. Church,et al.  Characterization of neuropeptide‐induced histamine release from human dispersed skin mast cells , 1988, British journal of pharmacology.

[60]  W F Taylor,et al.  Cutaneous laser-Doppler flowmetry: influence of underlying muscle blood flow. , 1988, Journal of applied physiology.

[61]  L. Ignarro,et al.  Mechanisms of endothelium-dependent vascular smooth muscle relaxation elicited by bradykinin and VIP. , 1987, The American journal of physiology.

[62]  P. Jansson,et al.  A microdialysis method allowing characterization of intercellular water space in humans. , 1987, The American journal of physiology.

[63]  Timothy J. Williams,et al.  Potent vasodilator activity of calcitonin gene-related peptide in human skin. , 1986, The Journal of investigative dermatology.

[64]  D. O'Leary,et al.  Effect of local warming on forearm reactive hyperaemia. , 1986, Clinical physiology.

[65]  J. Foreman,et al.  On the actions of substance P, somatostatin, and vasoactive intestinal polypeptide on rat peritoneal mast cells and in human skin , 1985, Naunyn-Schmiedeberg's Archives of Pharmacology.

[66]  M. Muley,et al.  Involvement of H1- and H2-receptors in triple response to histamine in human volunteers. , 1984, Indian journal of physiology and pharmacology.

[67]  W F Taylor,et al.  Effect of high local temperature on reflex cutaneous vasodilation. , 1984, Journal of applied physiology: respiratory, environmental and exercise physiology.

[68]  R. Saxena,et al.  Involvement of H1 & H2 receptors in histamine-induced skin response in human. , 1983, The Indian journal of medical research.

[69]  P. Anand,et al.  Topical capsaicin pretreatment inhibits axon reflex vasodilatation caused by somatostatin and vasoactive intestinal polypeptide in human skin , 1983, British journal of pharmacology.

[70]  M. Greaves,et al.  Histamine receptors in human skin: indirect evidence , 1982, The British journal of dermatology.

[71]  B. Fjellner,et al.  Studies on pruritogenic and histamine-releasing effects of some putative peptide neurotransmitters. , 1981, Acta dermato-venereologica.

[72]  M. Greaves,et al.  RECEPTORS FOR HISTAMINE IN HUMAN SKIN BLOOD VESSELS: A REVIEW , 1977, The British journal of dermatology.

[73]  L. Edvinsson,et al.  Immunohistochemical localization of a vasodilatory polypeptide (VIP) in cerebrovascular nerves , 1976, Brain Research.

[74]  V. Mutt,et al.  Potent Peripheral and Splanchnic Vasodilator Peptide from Normal Gut , 1970, Nature.

[75]  J. B.,et al.  The Blood Vessels of the Human Skin and Their Responses , 1928, Nature.

[76]  G. Hodges,et al.  The involvement of heating rate and vasoconstrictor nerves in the cutaneous vasodilator response to skin warming. , 2009, American journal of physiology. Heart and circulatory physiology.

[77]  F. Yamazaki,et al.  Rate dependency and role of nitric oxide in the vascular response to direct cooling in human skin. , 2006, Journal of applied physiology.

[78]  R. Henning,et al.  Vasoactive intestinal peptide: cardiovascular effects. , 2001, Cardiovascular research.

[79]  A. Jovanovic,et al.  Predominant role for nitric oxide in the relaxation induced by vasoactive intestinal polypeptide in human uterine artery. , 1998, Molecular human reproduction.

[80]  John M. Johnson,et al.  The Cutaneous Circulation , 1990 .

[81]  Oberg Pa,et al.  Laser-Doppler flowmetry. , 1990, Critical reviews in biomedical engineering.

[82]  T. Godfraind,et al.  Histamine receptors in the smooth muscle of human internal mammary artery and saphenous vein. , 1989, Pharmacology & toxicology.

[83]  C. Johnson,et al.  Effect of H1- and H2-receptor blockade on the inhibition of immediate cutaneous reactions. , 1984, Clinical pharmacy.

[84]  H M WRIGHT,et al.  THE CUTANEOUS CIRCULATION. , 1964, The Journal of osteopathy.