Effect of Synthetic Human Cholecystokinin‐33 on Pancreatic Blood Flow in Dogs

We have examined the effect of synthetic human cholecystokinin (CCK-33 and CCK-8) on pancreatic blood flow and protein output in anesthetized dogs. Human CCK-33 and CCK-8 increased pancreatic blood flow and protein output in a dose-related manner. There were no significant differences in increasing pancreatic blood flow between human CCK-33 and CCK-8, and increases in blood flow were closely related to the increase of the pancreatic enzyme secretion. L-364,718 (20 nmollkg) caused a potent inhibition of CCKstimulated pancreatic blood flow as well as protein output. The degree of inhibition by L-364,718 was dependent on the amount of CCK infused. This study demonstrates that increasing effect on pancreatic blood flow may be one of the biological actions of CCK mediated via CCK receptor. The CCK-33, one of longer molecular forms of CCK, is an important biological stimulator of pancreatic blood flow as well as of exocrine pancreatic secretion.

[1]  S. Sumi,et al.  Effects of synthetic human gastric inhibitory polypeptide on splanchnic circulation in dogs. , 1988, Gastroenterology.

[2]  S. Sumi,et al.  Studies on peptides. CLX. Synthesis of a 33-residue peptide corresponding to the entire amino acid sequence of human cholecystokinin (hCCK-33). , 1988, Chemical & pharmaceutical bulletin.

[3]  Kazutomo Inoue,et al.  Effects of synthetic kassinin on splanchnic circulation and exocrine pancreas in dogs , 1988, Peptides.

[4]  R. Hosotani,et al.  Effect of L364718, a New CCK Antagonist, on Amylase Secretion in Isolated Rat Pancreatic Acini , 1988, Pancreas.

[5]  K. A. Rich,et al.  Effects of L-364,718, a new cholecystokinin receptor antagonist, on camostate-induced growth of the rat pancreas. , 1988, Gastroenterology.

[6]  R. Hosotani,et al.  L364718, a new CCK antagonist, inhibits biological actions of CCK in conscious dogs , 1987, Peptides.

[7]  R. Hosotani,et al.  Synthetic Neuromedin C Stimulates Exocrine Pancreatic Secretion in Dogs and Rats , 1987, Pancreas.

[8]  R J Gould,et al.  In vivo pharmacology of L-364,718, a new potent nonpeptide peripheral cholecystokinin antagonist. , 1987, The Journal of pharmacology and experimental therapeutics.

[9]  R. Chang,et al.  Characterization of the binding of [3H]-(+/-)-L-364,718: a new potent, nonpeptide cholecystokinin antagonist radioligand selective for peripheral receptors. , 1986, Molecular pharmacology.

[10]  R. Chang,et al.  Biochemical and pharmacological characterization of an extremely potent and selective nonpeptide cholecystokinin antagonist. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Jansen,et al.  Physiological role and localization of cholecystokinin release in dogs. , 1986, The American journal of physiology.

[12]  J M Liesch,et al.  A potent nonpeptide cholecystokinin antagonist selective for peripheral tissues isolated from Aspergillus alliaceus. , 1985, Science.

[13]  Y. Hayashizaki,et al.  Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Elashoff,et al.  Bioactivity of cholecystokinin analogues: CCK-8 is not more potent than CCK-33. , 1984, American Journal of Physiology.

[15]  K. Malik,et al.  Effect of secretin and caerulein in canine pancreas: relation to prostaglandins. , 1983, The American journal of physiology.

[16]  R. Jensen,et al.  Interaction of cholecystokinin with specific membrane receptors on pancreatic acinar cells. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[17]  M. Korc,et al.  Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. , 1978, The American journal of physiology.

[18]  R L Bowman,et al.  Continuous measurement of tissue blood flow by laser-Doppler spectroscopy. , 1977, The American journal of physiology.

[19]  M. Grossman.  Physiological effects of gastrointestinal hormones. , 1977, Federation proceedings.

[20]  E. Rubinstein,et al.  Intestinal hormones in mesenteric vasodilation after intraduodenal agents. , 1972, The American journal of physiology.

[21]  H. S. Himal,et al.  Relationship between pancreatic secretion and pancreatic blood flow , 1970, Gut.

[22]  A. Hermreck,et al.  Metabolic and hemodynamic effects of secretin and pancreozymin on the pancreas. , 1970, Surgery.