Reduction of twin image in in-line digital holographic microscopy for biological cells' observation by segmentation mask method

During the reconstruction process of in-line digital holographic microscopic hologram, the main noise comes from the dispersed twin image. A segmentation mask method by selecting threshold value is introduced in order to reduce the disturbance. Experimental results show the satisfied effect by the proposed method.

[1]  Christian Depeursinge,et al.  Influence of shot noise on phase measurement accuracy in digital holographic microscopy. , 2007, Optics express.

[2]  Etienne Cuche,et al.  Submicrometer optical tomography by multiple-wavelength digital holographic microscopy. , 2006, Applied optics.

[3]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[4]  E. Cuche,et al.  Axial sub-nanometer accuracy in digital holographic microscopy , 2008 .

[5]  Florian Charriere,et al.  Shot noise influence in reconstructed phase images SNR in digital holographic microscopy , 2005, European Conference on Biomedical Optics.

[6]  Larbi Bouamama,et al.  Three-dimensional solid particle positions in a flow via multiangle off-axis digital holography. , 2008, Optics letters.

[7]  Christian Depeursinge,et al.  Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection. , 2009, Optics letters.

[8]  Emmett N. Leith,et al.  Wavefront Reconstruction with Continuous-Tone Objects* , 1963 .

[9]  I. Yamaguchi,et al.  Three-dimensional microscopy with phase-shifting digital holography. , 1998, Optics letters.

[10]  E. Cuche,et al.  Digital holography for quantitative phase-contrast imaging. , 1999, Optics letters.

[11]  E. Cuche,et al.  Spatial filtering for zero-order and twin-image elimination in digital off-axis holography. , 2000, Applied optics.

[12]  P. Scott,et al.  Phase retrieval and twin-image elimination for in-line Fresnel holograms , 1987 .

[13]  Etienne Cuche,et al.  Shot-noise influence on the reconstructed phase image signal-to-noise ratio in digital holographic microscopy. , 2006, Applied optics.

[14]  Werner P. O. Jueptner,et al.  Suppression of the dc term in digital holography , 1997 .

[15]  B. Kemper,et al.  Digital holographic microscopy for live cell applications and technical inspection. , 2008, Applied optics.

[16]  T. Latychevskaia,et al.  Solution to the twin image problem in holography. , 2006, Physical review letters.

[17]  G. L. Rogers,et al.  Elimination of the Unwanted Image in Diffraction Microscopy , 1951, Nature.

[18]  Michael Unser,et al.  Suppression of the zero-order term in off-axis digital holography through nonlinear filtering. , 2009, Applied optics.