Comparison of multi-sulfonamide biosensor immunoassays

Abstract Three different group-specific anti-sulfonamide antibodies were compared in inhibition assay formats in an optical biosensor (BIACORE 3000) using CM5 sensor chips coated with three different sulfonamide derivatives. The antibodies used were an anti-sulfamethazine monoclonal antibody (Mab) 21C7, the sulfonamide binding protein (SBP) in the Qflex Kit Sulfonamides and a recently developed mutant antibody (M.3.4). Each of these antibodies showed interactions with all 17 sulfonamides tested and one (Mab 21C7) was sensitive for the N 4 -acetyl metabolites also. The limits of detection of the different sulfonamides in chicken serum varied between 7 and >1000 ng ml −1 (Mab 21C7), 15 and 340 ng ml −1 (Qflex) and 4 and 82 ng ml −1 (Mutant M.3.4). The mutant M.3.4 based assay was found to be the most sensitive towards most of the sulfonamides whereas the Qflex Kit Sulfonamides detected the five sulfonamides registered for application in poultry in The Netherlands within the narrowest measurement range.

[1]  W. Haasnoot,et al.  Sulphonamide Antibodies: From Specific Polyclonals to Generic Monoclonals , 2000 .

[2]  M. Tuomola,et al.  Engineering of a broad specificity antibody for simultaneous detection of 13 sulfonamides at the maximum residue level. , 2004, Journal of agricultural and food chemistry.

[3]  E. Schacht,et al.  Generation of group-specific antibodies against sulfonamides. , 2003, Journal of agricultural and food chemistry.

[4]  F. Kohen,et al.  Generation of an Anti-idiotypic Antibody as a Surrogate Ligand for Sulfamethazine in Immunoassay Procedures , 2000 .

[5]  T. Vree,et al.  Plasma disposition and renal clearance of sulphadimidine and its metabolites in laying hens. , 1988, Research in veterinary science.

[6]  P. Hammer,et al.  Comparison of Biosensor, Microbiological, Immunochemical, and Physical Methods for Detection of Sulfamethazine Residues in Raw Milk. , 1996, Journal of food protection.

[7]  Christopher T. Elliott,et al.  On-line detection of sulfamethazine and sulfadiazine in porcine bile using a multi-channel high-throughput SPR biosensor , 2002 .

[8]  W. Haasnoot,et al.  Monoclonal Antibodies Against a Sulfathiazole Derivative for the Immunochemical Detection of Sulfonamides , 2000 .

[9]  W. Haasnoot,et al.  Application of an enzyme immunoassay for the determination of sulphamethazine (sulphadimidine) residues in swine urine and plasma and their use as predictors of the level in edible tissue. , 1996, Food additives and contaminants.

[10]  Christopher T. Elliott,et al.  The Development of a Rapid Immunobiosensor Screening Method for the Detection of Residues of Sulphadiazine , 1999 .

[11]  M. Tuomola,et al.  Further improvement of broad specificity hapten recognition with protein engineering. , 2003, Protein engineering.

[12]  A Sternesjö,et al.  Determination of sulfamethazine residues in milk by a surface plasmon resonance-based biosensor assay. , 1995, Analytical biochemistry.

[13]  M. Tuomola,et al.  Generic lanthanide fluoroimmunoassay for the simultaneous screening of 18 sulfonamides using an engineered antibody. , 2004, Analytical chemistry.

[14]  G. Wyatt,et al.  Molecular modeling of hapten structure and relevance to broad specificity immunoassay of sulfonamide antibiotics. , 1999, Bioconjugate chemistry.

[15]  C. V. Van Peteghem,et al.  Determination of eight sulfonamides in bovine kidney by liquid chromatography/tandem mass spectrometry with on-line extraction and sample clean-up. , 2000, Rapid communications in mass spectrometry : RCM.

[16]  Willem Haasnoot,et al.  Biosensor immunoassay for the detection of eight sulfonamides in chicken serum , 2003 .

[17]  Ursula Bilitewski,et al.  Detection of sulphamethazine with an optical biosensor and anti-idiotypic antibodies , 2000 .

[18]  W. Black,et al.  A rapid assay for detecting sulfonamides in tissues of slaughtered animals. , 2001, Journal of food protection.

[19]  C. Elliott,et al.  Comparison of porcine urine and bile as matrices to screen for the residues of two sulfonamides using a semi-automated enzyme immunoassay. , 1997, The Analyst.

[20]  G. Wyatt,et al.  Development of an ELISA for sulfachlorpyridazine and investigation of matrix effects from different sample extraction procedures , 2001, Food additives and contaminants.

[21]  S. Crooks,et al.  Immunobiosensor--an alternative to enzyme immunoassay screening for residues of two sulfonamides in pigs. , 1998, The Analyst.

[22]  N. Furusawa.  Determining the procedure for routine residue monitoring of sulfamethazine in edible animal tissues. , 2001, Biomedical chromatography : BMC.